The article discusses the enhancements and updates to the TRANSFAC® and TRANSCompel® databases, which are crucial for understanding gene regulation in eukaryotes. The databases have been improved with a new web interface, integrated versions of Match™ and Patch™, and extended links to other databases such as Ensembl, UniGene, EntrezGene, HumanPSD™, and TRANSPRO™. The content of the databases has been expanded, particularly with new data from Arabidopsis thaliana and Drosophila melanogaster. Automatic domain assignment for protein sequences using InterProScan, Pfam, SMART, and PROSITE has been implemented. The structure of TRANSCompel® has been significantly revised, introducing a separate table for detailed experimental evidence. The article also highlights the availability of the updated releases and the programs on the BIOBASE GmbH website.The article discusses the enhancements and updates to the TRANSFAC® and TRANSCompel® databases, which are crucial for understanding gene regulation in eukaryotes. The databases have been improved with a new web interface, integrated versions of Match™ and Patch™, and extended links to other databases such as Ensembl, UniGene, EntrezGene, HumanPSD™, and TRANSPRO™. The content of the databases has been expanded, particularly with new data from Arabidopsis thaliana and Drosophila melanogaster. Automatic domain assignment for protein sequences using InterProScan, Pfam, SMART, and PROSITE has been implemented. The structure of TRANSCompel® has been significantly revised, introducing a separate table for detailed experimental evidence. The article also highlights the availability of the updated releases and the programs on the BIOBASE GmbH website.