TREM2 macrophage promotes cardiac repair in myocardial infarction by reprogramming metabolism via SLC25A53

TREM2 macrophage promotes cardiac repair in myocardial infarction by reprogramming metabolism via SLC25A53

5 January 2024 | Shiyu Gong, Ming Zhai, Jiayun Shi, Guanye Yu, Zhijun Lei, Yefei Shi, Yanxi Zeng, Peinan Ju, Na Yang, Zhuo Zhang, Donghui Zhang, Jianhui Zhuang, Qing Yu, Xumin Zhang, Weixia Jian, Wei Wang, Wenhui Peng
This study investigates the role of TREM2-expressing macrophages in myocardial infarction (MI) repair. The authors found that macrophage-specific TREM2 deficiency worsened cardiac function and impaired post-MI repair. Through RNA-seq, protein, and molecular docking analyses, they identified that TREM2-expressing macrophages decreased SLC25A53 transcription via the SYK-SMAD4 signaling pathway after efferocytosis, which impaired NAD+ transport into mitochondria. This led to a breakpoint in the TCA cycle and increased itaconate production. In vitro experiments confirmed that itaconate secreted by TREM2+ macrophages inhibited cardiomyocyte apoptosis and promoted fibroblast proliferation. Conversely, overexpression of TREM2 in macrophages improved cardiac function. The study reveals a novel role for macrophage-specific TREM2 in MI, linking efferocytosis to immune metabolism during cardiac repair.This study investigates the role of TREM2-expressing macrophages in myocardial infarction (MI) repair. The authors found that macrophage-specific TREM2 deficiency worsened cardiac function and impaired post-MI repair. Through RNA-seq, protein, and molecular docking analyses, they identified that TREM2-expressing macrophages decreased SLC25A53 transcription via the SYK-SMAD4 signaling pathway after efferocytosis, which impaired NAD+ transport into mitochondria. This led to a breakpoint in the TCA cycle and increased itaconate production. In vitro experiments confirmed that itaconate secreted by TREM2+ macrophages inhibited cardiomyocyte apoptosis and promoted fibroblast proliferation. Conversely, overexpression of TREM2 in macrophages improved cardiac function. The study reveals a novel role for macrophage-specific TREM2 in MI, linking efferocytosis to immune metabolism during cardiac repair.
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