This supplementary material provides detailed supplementary figures and tables to support the main findings of the article "TRPV3 activation by different agonists accompanied by lipid dissociation from the vanilloid site" by Kirill D. Nadezhdin et al. The content includes: - **Figures S1 to S7**: These figures cover the workflow for cryo-EM data processing for various structures of TRPV3, including the APO structure, TRPV3 in the presence of THCV, and TRPV3 in the presence of 2-APB. They also include comparisons of pore structures in open-state TRPV3 and detailed views of the 2-APB-bound states. - **Table S1**: This table provides statistics on cryo-EM data collection, refinement, and validation. - **Table S2**: This table lists the EC50 values for TRPV3 activation by THCV and 2-APB, along with the number of independent measurements. The material aims to provide comprehensive insights into the structural and functional aspects of TRPV3 activation by different agonists, particularly focusing on lipid dissociation from the vanilloid site.This supplementary material provides detailed supplementary figures and tables to support the main findings of the article "TRPV3 activation by different agonists accompanied by lipid dissociation from the vanilloid site" by Kirill D. Nadezhdin et al. The content includes: - **Figures S1 to S7**: These figures cover the workflow for cryo-EM data processing for various structures of TRPV3, including the APO structure, TRPV3 in the presence of THCV, and TRPV3 in the presence of 2-APB. They also include comparisons of pore structures in open-state TRPV3 and detailed views of the 2-APB-bound states. - **Table S1**: This table provides statistics on cryo-EM data collection, refinement, and validation. - **Table S2**: This table lists the EC50 values for TRPV3 activation by THCV and 2-APB, along with the number of independent measurements. The material aims to provide comprehensive insights into the structural and functional aspects of TRPV3 activation by different agonists, particularly focusing on lipid dissociation from the vanilloid site.