Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

July 8, 2010 | Michelle Christian, Tomas Cermak, Erin L. Doyle, Clarice Schmidt, Feng Zhang, Aaron Hummel, Adam J. Bogdanove, and Daniel F. Voytas
The article introduces a new class of sequence-specific nucleases called Transcription Activator-Like Effector Nucleases (TALENs), which are created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. TALENs can direct DNA double-strand breaks to specific, targeted sites in the genome. The authors tested the activity of TALENs using a yeast assay and found that they could effectively cleave DNA targets with different spacer lengths. They also demonstrated that custom TALENs designed to recognize novel chromosomal DNA sequences were functional, showing promise for targeted genome modification. However, some custom TALENs failed to function, suggesting that unknown rules govern the assembly of functional repeat domains. The study highlights the potential of TALENs for sequence-specific genome editing but also identifies areas for further research to optimize their design and function.The article introduces a new class of sequence-specific nucleases called Transcription Activator-Like Effector Nucleases (TALENs), which are created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. TALENs can direct DNA double-strand breaks to specific, targeted sites in the genome. The authors tested the activity of TALENs using a yeast assay and found that they could effectively cleave DNA targets with different spacer lengths. They also demonstrated that custom TALENs designed to recognize novel chromosomal DNA sequences were functional, showing promise for targeted genome modification. However, some custom TALENs failed to function, suggesting that unknown rules govern the assembly of functional repeat domains. The study highlights the potential of TALENs for sequence-specific genome editing but also identifies areas for further research to optimize their design and function.
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Understanding Targeting DNA Double-Strand Breaks with TAL Effector Nucleases