19 February 2013 | Ali Khoddami, Meredith A. Wilkes and Thomas H. Roberts
This review discusses techniques for analyzing plant phenolic compounds, focusing on extraction, identification, and quantification methods. Phenolic compounds, including simple phenols, benzoic acids, cinnamic acids, coumarins, tannins, lignins, lignans, and flavonoids, are abundant in plant-based foods and have various health benefits. Extraction methods such as organic solvent extraction, spectrophotometric, and chromatographic techniques are used to identify and quantify these compounds. The review highlights the importance of sample preparation, including drying, milling, and defatting, to ensure effective extraction. Various solvents, such as water, ethanol, and acetone, are used for extraction, with optimal conditions varying depending on the plant source. Modern extraction techniques like ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), supercritical fluid extraction (SFE), and subcritical water extraction (SCWE) are also discussed, as they offer faster and more efficient extraction with reduced use of organic solvents. Quantification methods include spectrophotometric assays, gas chromatography (GC), and high-performance liquid chromatography (HPLC), which are used to measure the concentration of phenolic compounds. The review emphasizes the need for accurate and efficient methods to analyze phenolic compounds in plant-based products for their potential health benefits.This review discusses techniques for analyzing plant phenolic compounds, focusing on extraction, identification, and quantification methods. Phenolic compounds, including simple phenols, benzoic acids, cinnamic acids, coumarins, tannins, lignins, lignans, and flavonoids, are abundant in plant-based foods and have various health benefits. Extraction methods such as organic solvent extraction, spectrophotometric, and chromatographic techniques are used to identify and quantify these compounds. The review highlights the importance of sample preparation, including drying, milling, and defatting, to ensure effective extraction. Various solvents, such as water, ethanol, and acetone, are used for extraction, with optimal conditions varying depending on the plant source. Modern extraction techniques like ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), supercritical fluid extraction (SFE), and subcritical water extraction (SCWE) are also discussed, as they offer faster and more efficient extraction with reduced use of organic solvents. Quantification methods include spectrophotometric assays, gas chromatography (GC), and high-performance liquid chromatography (HPLC), which are used to measure the concentration of phenolic compounds. The review emphasizes the need for accurate and efficient methods to analyze phenolic compounds in plant-based products for their potential health benefits.