The BioGRID interaction database, available at http://thebiogrid.org, is an open-access resource that curates genetic and protein interactions from the primary biomedical literature for major model organisms and humans. As of September 2014, BioGRID contains 749,912 interactions from 43,149 publications across 30 model organisms, representing a 50% increase since the 2013 update. The database is freely distributed through partner model organism databases and meta-databases, and can be downloaded in various formats. BioGRID curates interactions for model yeasts *Saccharomyces cerevisiae* and *Schizosaccharomyces pombe*, as well as over 30 other model organisms. The curation process involves manual and semi-automated text-mining approaches to identify relevant publications and curate interaction data using structured evidence codes and phenotype ontologies. The BioGRID architecture has been improved to support a broader range of interaction types, complex multi-gene/protein interactions, and post-translational modifications. Themed curation projects focus on specific biological processes or diseases, such as the ubiquitin-proteasome system and arachidonic acid pathway. The database's web interface allows users to search and download interaction data, and it is accessible through various biological databases and software tools. Future developments will include further improvements to the curation management system, expanded thematic projects, and enhanced computational approaches for tissue- and disease-specific functional networks.The BioGRID interaction database, available at http://thebiogrid.org, is an open-access resource that curates genetic and protein interactions from the primary biomedical literature for major model organisms and humans. As of September 2014, BioGRID contains 749,912 interactions from 43,149 publications across 30 model organisms, representing a 50% increase since the 2013 update. The database is freely distributed through partner model organism databases and meta-databases, and can be downloaded in various formats. BioGRID curates interactions for model yeasts *Saccharomyces cerevisiae* and *Schizosaccharomyces pombe*, as well as over 30 other model organisms. The curation process involves manual and semi-automated text-mining approaches to identify relevant publications and curate interaction data using structured evidence codes and phenotype ontologies. The BioGRID architecture has been improved to support a broader range of interaction types, complex multi-gene/protein interactions, and post-translational modifications. Themed curation projects focus on specific biological processes or diseases, such as the ubiquitin-proteasome system and arachidonic acid pathway. The database's web interface allows users to search and download interaction data, and it is accessible through various biological databases and software tools. Future developments will include further improvements to the curation management system, expanded thematic projects, and enhanced computational approaches for tissue- and disease-specific functional networks.