The article discusses the sequencing of the euchromatic part of human chromosome 22, a significant milestone in the Human Genome Project. The sequence spans 33.4 megabases, contains at least 545 genes and 134 pseudogenes, and provides insights into the complex chromosomal landscapes of the human genome. Two approaches to genome sequencing are mentioned: the clone-by-clone method, which is used in the public genome project, and the whole-genome shotgun method. Chromosome 22 was chosen for sequencing due to its relatively small size and the availability of a high-resolution map, as well as its association with several genetic disorders. The sequencing process involved constructing clone maps using various genomic clones and assembling them into contiguous sequences. Despite efforts, some gaps remained, likely due to unclonable sequences. The analysis of the sequence revealed a high density of repetitive elements, with over 41.9% of the sequence comprising interspersed and tandem repeats. The study also identified gene families, pseudogenes, and CpG islands, highlighting the complexity of the chromosome's genetic content. The article emphasizes the importance of the chromosome 22 sequence in understanding gene function, identifying disease-related genes, and studying evolutionary relationships. It also discusses the challenges in gene prediction and the need for experimental validation. The availability of the sequence has benefited research on genetic disorders and has provided a foundation for further studies on the human genome. The study concludes that the clone-by-clone strategy is effective for generating a complete genomic sequence and that the sequence of chromosome 22 offers a glimpse into the information that will be revealed from the remaining chromosomes.The article discusses the sequencing of the euchromatic part of human chromosome 22, a significant milestone in the Human Genome Project. The sequence spans 33.4 megabases, contains at least 545 genes and 134 pseudogenes, and provides insights into the complex chromosomal landscapes of the human genome. Two approaches to genome sequencing are mentioned: the clone-by-clone method, which is used in the public genome project, and the whole-genome shotgun method. Chromosome 22 was chosen for sequencing due to its relatively small size and the availability of a high-resolution map, as well as its association with several genetic disorders. The sequencing process involved constructing clone maps using various genomic clones and assembling them into contiguous sequences. Despite efforts, some gaps remained, likely due to unclonable sequences. The analysis of the sequence revealed a high density of repetitive elements, with over 41.9% of the sequence comprising interspersed and tandem repeats. The study also identified gene families, pseudogenes, and CpG islands, highlighting the complexity of the chromosome's genetic content. The article emphasizes the importance of the chromosome 22 sequence in understanding gene function, identifying disease-related genes, and studying evolutionary relationships. It also discusses the challenges in gene prediction and the need for experimental validation. The availability of the sequence has benefited research on genetic disorders and has provided a foundation for further studies on the human genome. The study concludes that the clone-by-clone strategy is effective for generating a complete genomic sequence and that the sequence of chromosome 22 offers a glimpse into the information that will be revealed from the remaining chromosomes.