The Dartmouth Stellar Evolution Database is a comprehensive collection of stellar evolution tracks and isochrones, covering a wide range of metallicities ([Fe/H]) from -2.5 to +0.5, α-element enhancements ([α/Fe]) from -0.2 to +0.8 (for [Fe/H] ≤0) or +0.2 (for [Fe/H] >0), and initial helium mass fractions (Y) from 0.245 to 0.40. The database includes tracks for masses between 0.1 and 4 M⊙, allowing isochrones to be generated for ages as young as 250 Myr. The tracks and isochrones have been transformed to various photometric systems, including standard UBV(RI)$_C$, Strömgren uvby, Sloan Digital Sky Survey ugriz, Two Micron All Sky Survey JHK$_s$, and Hubble Space Telescope ACS-WFC and WFPC2. The database is accessible through a website, providing all tracks, isochrones, and supporting computer programs. The paper discusses the physics employed in the Dartmouth Stellar Evolution Program (DSEP), the model grids, and the color-T$_{\text{eff}}$ transformations. It also compares the DSEP models to other models and observational data from the literature, demonstrating their performance in fitting observed color-magnitude diagrams (CMDs) of open clusters and a globular cluster. The synthetic colors are found to perform well in bandpasses equivalent to V or redder but are consistently too red in bluer bands. The database is expected to be useful for studies of resolved stellar populations in the local Universe.The Dartmouth Stellar Evolution Database is a comprehensive collection of stellar evolution tracks and isochrones, covering a wide range of metallicities ([Fe/H]) from -2.5 to +0.5, α-element enhancements ([α/Fe]) from -0.2 to +0.8 (for [Fe/H] ≤0) or +0.2 (for [Fe/H] >0), and initial helium mass fractions (Y) from 0.245 to 0.40. The database includes tracks for masses between 0.1 and 4 M⊙, allowing isochrones to be generated for ages as young as 250 Myr. The tracks and isochrones have been transformed to various photometric systems, including standard UBV(RI)$_C$, Strömgren uvby, Sloan Digital Sky Survey ugriz, Two Micron All Sky Survey JHK$_s$, and Hubble Space Telescope ACS-WFC and WFPC2. The database is accessible through a website, providing all tracks, isochrones, and supporting computer programs. The paper discusses the physics employed in the Dartmouth Stellar Evolution Program (DSEP), the model grids, and the color-T$_{\text{eff}}$ transformations. It also compares the DSEP models to other models and observational data from the literature, demonstrating their performance in fitting observed color-magnitude diagrams (CMDs) of open clusters and a globular cluster. The synthetic colors are found to perform well in bandpasses equivalent to V or redder but are consistently too red in bluer bands. The database is expected to be useful for studies of resolved stellar populations in the local Universe.