The EMBL-EBI Job Dispatcher framework, launched in 2009, provides free access to a range of bioinformatics tools for sequence analysis. These tools include sequence similarity search services (BLAST, FASTA, PSI-Search), multiple sequence alignment tools (Clustal Omega, MAFFT, T-Coffee), and other sequence analysis tools (InterProScan). The framework supports users through a uniform web interface and Web Services, enabling them to search mainstream sequence databases like ENA, UniProt, and Ensembl Genomes. New tools and databases, such as NCBI BLAST+, InterProScan 5, PfamScan, RNA analysis tools, and non-coding RNA databases, have been integrated, while deprecated services have been retired. The framework also offers interactive workflows, enhanced result representations, and robust Web Services APIs. User feedback and collaboration with the bioinformatics community drive continuous improvements, ensuring the framework remains relevant and useful for modern biological research.The EMBL-EBI Job Dispatcher framework, launched in 2009, provides free access to a range of bioinformatics tools for sequence analysis. These tools include sequence similarity search services (BLAST, FASTA, PSI-Search), multiple sequence alignment tools (Clustal Omega, MAFFT, T-Coffee), and other sequence analysis tools (InterProScan). The framework supports users through a uniform web interface and Web Services, enabling them to search mainstream sequence databases like ENA, UniProt, and Ensembl Genomes. New tools and databases, such as NCBI BLAST+, InterProScan 5, PfamScan, RNA analysis tools, and non-coding RNA databases, have been integrated, while deprecated services have been retired. The framework also offers interactive workflows, enhanced result representations, and robust Web Services APIs. User feedback and collaboration with the bioinformatics community drive continuous improvements, ensuring the framework remains relevant and useful for modern biological research.