The EMBL-EBI Job Dispatcher framework provides free access to a range of bioinformatics tools for sequence analysis since 2009. These tools include sequence similarity search services like BLAST, FASTA, and PSI-Search, multiple sequence alignment tools like Clustal Omega, MAFFT, and T-Coffee, and other sequence analysis tools like InterProScan. Users can search mainstream sequence databases such as ENA, UniProt, and Ensembl Genomes through a uniform web interface or via Web Services using common programming languages. Integration with EBI Search and dbfetch further enhances the framework's utility. New tools and databases, such as NCBI BLAST+, InterProScan 5, PfamScan, RNA analysis tools, and new databases like ENA non-coding and WormBase ParaSite, ensure the framework remains relevant. Deprecated services are retired to maintain relevance. The framework is used by academic and industry scientists, handling around 110 million analysis jobs in 2014. Help pages, tutorials, and user guides are available, along with training courses and helpdesk support. Continued feedback and collaboration with bioinformatics tools and data providers help improve the framework's accessibility and quality. The EMBL-EBI Job Dispatcher is a modular and configuration-driven framework for both novice and expert users. It offers a uniform web interface for uploading or selecting data for analysis in various applications. Input validation and parameter help are built-in, and results are enriched with data from other applications. SOAP and REST Web Services provide stable APIs for programmatic use. New functionalities include interactive workflows for moving between tool categories and constructing phylogenetic trees from sequence similarity search results. New result representations include user-friendly web interfaces, downloadable result summaries, and new output formats for phylogenetic analysis. The framework has been used by academic and industry users for almost 260 million analysis jobs since 2009. Web Services are particularly useful for integration into third-party pipelines. Future developments include integrating new tools like HMMER 3 and R-Coffee, and new data resources like ENA Barcode and Geospatial databases. Additional support for users will include webinars and video-based tutorials. The framework continues to evolve to meet the needs of the biological community.The EMBL-EBI Job Dispatcher framework provides free access to a range of bioinformatics tools for sequence analysis since 2009. These tools include sequence similarity search services like BLAST, FASTA, and PSI-Search, multiple sequence alignment tools like Clustal Omega, MAFFT, and T-Coffee, and other sequence analysis tools like InterProScan. Users can search mainstream sequence databases such as ENA, UniProt, and Ensembl Genomes through a uniform web interface or via Web Services using common programming languages. Integration with EBI Search and dbfetch further enhances the framework's utility. New tools and databases, such as NCBI BLAST+, InterProScan 5, PfamScan, RNA analysis tools, and new databases like ENA non-coding and WormBase ParaSite, ensure the framework remains relevant. Deprecated services are retired to maintain relevance. The framework is used by academic and industry scientists, handling around 110 million analysis jobs in 2014. Help pages, tutorials, and user guides are available, along with training courses and helpdesk support. Continued feedback and collaboration with bioinformatics tools and data providers help improve the framework's accessibility and quality. The EMBL-EBI Job Dispatcher is a modular and configuration-driven framework for both novice and expert users. It offers a uniform web interface for uploading or selecting data for analysis in various applications. Input validation and parameter help are built-in, and results are enriched with data from other applications. SOAP and REST Web Services provide stable APIs for programmatic use. New functionalities include interactive workflows for moving between tool categories and constructing phylogenetic trees from sequence similarity search results. New result representations include user-friendly web interfaces, downloadable result summaries, and new output formats for phylogenetic analysis. The framework has been used by academic and industry users for almost 260 million analysis jobs since 2009. Web Services are particularly useful for integration into third-party pipelines. Future developments include integrating new tools like HMMER 3 and R-Coffee, and new data resources like ENA Barcode and Geospatial databases. Additional support for users will include webinars and video-based tutorials. The framework continues to evolve to meet the needs of the biological community.