The EMBL Nucleotide Sequence Database (EMBL) is a comprehensive collection of nucleotide sequences and annotations from public sources, maintained at the European Bioinformatics Institute (EBI) near Cambridge, UK. It is part of an international collaboration with DDBJ (Japan) and GenBank (USA). Data are exchanged daily between the collaborating institutes to ensure synchronization. Webin is the preferred tool for submitting nucleotide sequences, including Third Party Annotation (TPA) and alignments. Automated procedures are provided for submissions from large-scale sequencing projects and the European Patent Office. New and updated data records are distributed daily, and the entire database is released four times a year. Access to the sequence data is provided via ftp and several WWW interfaces. The web-based Sequence Retrieval System (SRS) allows linking nucleotide data to other molecular biology databases at the EBI. Other tools are available for sequence similarity searching, such as FASTA and BLAST. Over the past year, the EMBL Nucleotide Sequence Database has increased in size from 27.2 million entries to 42.3 million entries, with 4.4 million being Whole Genome Shotgun (WGS) data. The sequence length limit has been removed, the EMBLCDSs dataset has been launched, and the functionality of the Sequence Version Archive has been extended. Other databases provided by the EBI include UniProt, InterPro, E-MSD, Ensembl, Genome Reviews, IntEnz, and IntAct. Submissions to the EMBL Nucleotide Sequence Database can be made via Webin, Webin-Align, or email/ftp for large-scale projects. WGS data submissions are handled on a one-by-one basis. Entries can be updated via the update form. Data are grouped into divisions based on methodology or taxonomic origin. WGS data are part of EMBL (Release) and EMBL (Updates). The EMBLCDSs dataset contains all coding sequences annotated in EMBL entries. The EMBL Sequence Version Archive (SVA) is a repository of all versions of entries. Completed genome sequences are available via the EBI Genomes server. Sequence searching tools include FASTA and WU-BLAST. The EMBL Nucleotide Sequence Database is cited as Kanz, C. et al. (2005). Contact information is provided for data submissions and other inquiries.The EMBL Nucleotide Sequence Database (EMBL) is a comprehensive collection of nucleotide sequences and annotations from public sources, maintained at the European Bioinformatics Institute (EBI) near Cambridge, UK. It is part of an international collaboration with DDBJ (Japan) and GenBank (USA). Data are exchanged daily between the collaborating institutes to ensure synchronization. Webin is the preferred tool for submitting nucleotide sequences, including Third Party Annotation (TPA) and alignments. Automated procedures are provided for submissions from large-scale sequencing projects and the European Patent Office. New and updated data records are distributed daily, and the entire database is released four times a year. Access to the sequence data is provided via ftp and several WWW interfaces. The web-based Sequence Retrieval System (SRS) allows linking nucleotide data to other molecular biology databases at the EBI. Other tools are available for sequence similarity searching, such as FASTA and BLAST. Over the past year, the EMBL Nucleotide Sequence Database has increased in size from 27.2 million entries to 42.3 million entries, with 4.4 million being Whole Genome Shotgun (WGS) data. The sequence length limit has been removed, the EMBLCDSs dataset has been launched, and the functionality of the Sequence Version Archive has been extended. Other databases provided by the EBI include UniProt, InterPro, E-MSD, Ensembl, Genome Reviews, IntEnz, and IntAct. Submissions to the EMBL Nucleotide Sequence Database can be made via Webin, Webin-Align, or email/ftp for large-scale projects. WGS data submissions are handled on a one-by-one basis. Entries can be updated via the update form. Data are grouped into divisions based on methodology or taxonomic origin. WGS data are part of EMBL (Release) and EMBL (Updates). The EMBLCDSs dataset contains all coding sequences annotated in EMBL entries. The EMBL Sequence Version Archive (SVA) is a repository of all versions of entries. Completed genome sequences are available via the EBI Genomes server. Sequence searching tools include FASTA and WU-BLAST. The EMBL Nucleotide Sequence Database is cited as Kanz, C. et al. (2005). Contact information is provided for data submissions and other inquiries.