The EMBL Nucleotide Sequence Database, maintained at the European Bioinformatics Institute (EBI) near Cambridge, UK, is a comprehensive resource for nucleotide sequences and annotations. It is part of an international collaboration with DDBJ (Japan) and GenBank (USA), with daily data exchange to ensure synchronization. The database accepts submissions via Webin, a preferred tool for individual submissions, and supports large-scale sequencing projects and data from the European Patent Office. New and updated records are distributed daily, and the entire database is released four times a year. Access is provided through FTP, WWW interfaces, and the web-based Sequence Retrieval System (SRS), which links to other molecular biology databases. Recent developments include removing the sequence length limit, launching the EMBLDCSs dataset, extending the Sequence Version Archive functionality, and revising quality rules for Third Party Annotation (TPA) data. The database has grown from 27.2 million entries in September 2003 to 42.3 million entries in September 2004, with over 185,000 organisms represented. Submissions are essential for ensuring public availability and computational accessibility, and the database supports various tools for sequence similarity searching, such as FASTA and BLAST.The EMBL Nucleotide Sequence Database, maintained at the European Bioinformatics Institute (EBI) near Cambridge, UK, is a comprehensive resource for nucleotide sequences and annotations. It is part of an international collaboration with DDBJ (Japan) and GenBank (USA), with daily data exchange to ensure synchronization. The database accepts submissions via Webin, a preferred tool for individual submissions, and supports large-scale sequencing projects and data from the European Patent Office. New and updated records are distributed daily, and the entire database is released four times a year. Access is provided through FTP, WWW interfaces, and the web-based Sequence Retrieval System (SRS), which links to other molecular biology databases. Recent developments include removing the sequence length limit, launching the EMBLDCSs dataset, extending the Sequence Version Archive functionality, and revising quality rules for Third Party Annotation (TPA) data. The database has grown from 27.2 million entries in September 2003 to 42.3 million entries in September 2004, with over 185,000 organisms represented. Submissions are essential for ensuring public availability and computational accessibility, and the database supports various tools for sequence similarity searching, such as FASTA and BLAST.