The ENZYME database, maintained by the Swiss Institute of Bioinformatics, is a comprehensive repository of enzyme nomenclature information. As of 2000, it contains data on 3,705 enzymes and is accessible through the ExPASy WWW server. The database is primarily based on the recommendations of the International Union of Biochemistry and Molecular Biology (IUBMB) and includes EC numbers, enzyme descriptions, reaction mechanisms, and related literature references. It is widely used in the development of metabolic databases and software for querying and visualizing metabolic pathways. The ENZYME database is structured to be compatible with SWISS-PROT and EMBL sequence databases, facilitating efficient updates and corrections. The current release (25.0) requires approximately 1.5 MB of disk space and is distributed with user manuals and other supporting files. Users can access the database interactively via the ExPASy server and submit new data or updates to the IUBMB Nomenclature Committee.The ENZYME database, maintained by the Swiss Institute of Bioinformatics, is a comprehensive repository of enzyme nomenclature information. As of 2000, it contains data on 3,705 enzymes and is accessible through the ExPASy WWW server. The database is primarily based on the recommendations of the International Union of Biochemistry and Molecular Biology (IUBMB) and includes EC numbers, enzyme descriptions, reaction mechanisms, and related literature references. It is widely used in the development of metabolic databases and software for querying and visualizing metabolic pathways. The ENZYME database is structured to be compatible with SWISS-PROT and EMBL sequence databases, facilitating efficient updates and corrections. The current release (25.0) requires approximately 1.5 MB of disk space and is distributed with user manuals and other supporting files. Users can access the database interactively via the ExPASy server and submit new data or updates to the IUBMB Nomenclature Committee.