This systematic review explores the efficacy of stem cell therapy in enhancing wound healing, particularly in chronic wounds. The review comprehensively examines clinical, translational, and primary literature, focusing on various sources of stem cells such as endothelial progenitor cells, bone marrow-derived mesenchymal stem cells (BM-MSCs), adipose tissue-derived mesenchymal stem cells (AD-MSCs), and inducible pluripotent stem cells (iPSCs). Key findings include: 1. **Adipose Tissue-Derived Mesenchymal Stem Cells (AD-MSCs)**: AD-MSCs are highlighted as an optimal choice due to their abundant supply, easy isolation, and pro-angiogenic factor secretion. They have shown efficacy in treating various conditions, including peripheral arterial disease, diabetic wounds, hypertensive ulcers, and venous ulcers. Delivery methods include topical application, scaffold incorporation, and combination with plasma-rich proteins. 2. **Skin-Derived Stem Cells**: Autologous keratinocytes and hair follicle-derived stem cells have demonstrated effectiveness in wound healing, with studies showing significant improvements in wound closure rates and reduced healing times. 3. **Peripheral Blood-Derived Cells/Endothelial Progenitor Stem Cells (EPCs)**: EPCs are involved in revascularization and tissue repair, with studies showing their ability to stimulate angiogenesis and promote wound healing. 4. **Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSCs)**: BM-MSCs have been shown to enhance wound healing by differentiating into various cell types and releasing proangiogenic factors. Studies have reported significant reductions in wound area and increased vascularity. 5. **Inducible Pluripotent Stem Cells (iPSCs)**: iPSCs have the potential to differentiate into all skin cell types and may be useful in wound healing, but safety concerns regarding teratoma formation need further investigation. The review concludes that stem cell therapy represents a promising therapeutic avenue for chronic wounds, offering potential benefits in reducing healthcare costs and improving patient quality of life. However, additional research is needed to fully understand the molecular mechanisms underlying the effects of stem cells in wound healing.This systematic review explores the efficacy of stem cell therapy in enhancing wound healing, particularly in chronic wounds. The review comprehensively examines clinical, translational, and primary literature, focusing on various sources of stem cells such as endothelial progenitor cells, bone marrow-derived mesenchymal stem cells (BM-MSCs), adipose tissue-derived mesenchymal stem cells (AD-MSCs), and inducible pluripotent stem cells (iPSCs). Key findings include: 1. **Adipose Tissue-Derived Mesenchymal Stem Cells (AD-MSCs)**: AD-MSCs are highlighted as an optimal choice due to their abundant supply, easy isolation, and pro-angiogenic factor secretion. They have shown efficacy in treating various conditions, including peripheral arterial disease, diabetic wounds, hypertensive ulcers, and venous ulcers. Delivery methods include topical application, scaffold incorporation, and combination with plasma-rich proteins. 2. **Skin-Derived Stem Cells**: Autologous keratinocytes and hair follicle-derived stem cells have demonstrated effectiveness in wound healing, with studies showing significant improvements in wound closure rates and reduced healing times. 3. **Peripheral Blood-Derived Cells/Endothelial Progenitor Stem Cells (EPCs)**: EPCs are involved in revascularization and tissue repair, with studies showing their ability to stimulate angiogenesis and promote wound healing. 4. **Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSCs)**: BM-MSCs have been shown to enhance wound healing by differentiating into various cell types and releasing proangiogenic factors. Studies have reported significant reductions in wound area and increased vascularity. 5. **Inducible Pluripotent Stem Cells (iPSCs)**: iPSCs have the potential to differentiate into all skin cell types and may be useful in wound healing, but safety concerns regarding teratoma formation need further investigation. The review concludes that stem cell therapy represents a promising therapeutic avenue for chronic wounds, offering potential benefits in reducing healthcare costs and improving patient quality of life. However, additional research is needed to fully understand the molecular mechanisms underlying the effects of stem cells in wound healing.