The Gene Ontology (GO) Consortium has made significant advancements in the past two years, enhancing the Gene Ontology resource, which is the most comprehensive source of computable knowledge about gene functions. Key improvements include the development of the GO-CAM annotation framework, formalized with a computational schema to validate and check the growing repository of 2838 GO-CAMs. Collaborations with various organizations have led to a 10% increase in GO annotations, a 25% increase in annotated gene products, and over 9,400 new scientific articles annotated. The consortium has also reviewed 20,000 experimental annotations, corresponding to 2.5% of all experimental GO annotations, to ensure consistency and accuracy. The website (http://geneontology.org) has been redesigned for better access to documentation, downloads, and tools, and a historical archive covering 15 years of GO data is available. Additionally, the consortium has improved the GO ontology, refined taxon constraints, leveraged inter-ontology inferences, and enhanced the GO toolset, including the Noctua curation platform and the GO API. These efforts aim to maintain the accuracy and usability of the GO resource, supporting traceability and reproducibility in genomic and biomedical research.The Gene Ontology (GO) Consortium has made significant advancements in the past two years, enhancing the Gene Ontology resource, which is the most comprehensive source of computable knowledge about gene functions. Key improvements include the development of the GO-CAM annotation framework, formalized with a computational schema to validate and check the growing repository of 2838 GO-CAMs. Collaborations with various organizations have led to a 10% increase in GO annotations, a 25% increase in annotated gene products, and over 9,400 new scientific articles annotated. The consortium has also reviewed 20,000 experimental annotations, corresponding to 2.5% of all experimental GO annotations, to ensure consistency and accuracy. The website (http://geneontology.org) has been redesigned for better access to documentation, downloads, and tools, and a historical archive covering 15 years of GO data is available. Additionally, the consortium has improved the GO ontology, refined taxon constraints, leveraged inter-ontology inferences, and enhanced the GO toolset, including the Noctua curation platform and the GO API. These efforts aim to maintain the accuracy and usability of the GO resource, supporting traceability and reproducibility in genomic and biomedical research.