The Genotype-Tissue Expression (GTEx) project aims to establish a resource database and tissue bank to study the relationship between genetic variation and gene expression in human tissues. This project addresses the gap in understanding the mechanisms behind disease susceptibility, as many associated variants are not linked to protein-coding changes but may instead influence gene regulation. The GTEx project collects biospecimens from a wide range of tissues, including those that are difficult to obtain from living donors, such as brain, heart, and pancreas. The project involves a large-scale collaboration between multiple institutions and aims to provide a comprehensive resource for the scientific community to study gene expression and its regulation across various tissues. The GTEx project includes a pilot phase that tested the feasibility of establishing a rapid autopsy program to collect high-quality nucleic acids and gene expression data. The project is now scaling up to include approximately 900 post-mortem donors by the end of 2015. The project collects biospecimens from donors who are aged 21–70 and have a low post-mortem interval. Donors are eligible if they do not have certain medical conditions, such as HIV, viral hepatitis, or metastatic cancer. The project collects various biospecimens, including blood, skin, and brain tissues, and uses these to study gene expression and other molecular phenotypes. The GTEx project also includes a Brain Bank for the collection and analysis of brain tissues, and a Laboratory, Data Analysis, and Coordinating Center (LDACC) for nucleic acid extractions, DNA genotyping, and RNA expression analysis. The project uses various technologies, including RNA sequencing and microarrays, to measure gene expression and identify expression quantitative trait loci (eQTLs). The project also includes a statistical analysis component to develop improved methods for analyzing eQTL data. The GTEx project aims to provide a comprehensive resource for the scientific community to study the relationship between genetic variation and gene expression across multiple tissues. The project includes a data release policy that ensures the rapid sharing of data while balancing the risks of identifying donors. The project also includes an ethical, legal, and social implications (ELSI) component to address the potential risks and benefits of donor recruitment and consent. The GTEx project is a large-scale initiative that aims to advance the understanding of genetic variation and gene regulation in human tissues.The Genotype-Tissue Expression (GTEx) project aims to establish a resource database and tissue bank to study the relationship between genetic variation and gene expression in human tissues. This project addresses the gap in understanding the mechanisms behind disease susceptibility, as many associated variants are not linked to protein-coding changes but may instead influence gene regulation. The GTEx project collects biospecimens from a wide range of tissues, including those that are difficult to obtain from living donors, such as brain, heart, and pancreas. The project involves a large-scale collaboration between multiple institutions and aims to provide a comprehensive resource for the scientific community to study gene expression and its regulation across various tissues. The GTEx project includes a pilot phase that tested the feasibility of establishing a rapid autopsy program to collect high-quality nucleic acids and gene expression data. The project is now scaling up to include approximately 900 post-mortem donors by the end of 2015. The project collects biospecimens from donors who are aged 21–70 and have a low post-mortem interval. Donors are eligible if they do not have certain medical conditions, such as HIV, viral hepatitis, or metastatic cancer. The project collects various biospecimens, including blood, skin, and brain tissues, and uses these to study gene expression and other molecular phenotypes. The GTEx project also includes a Brain Bank for the collection and analysis of brain tissues, and a Laboratory, Data Analysis, and Coordinating Center (LDACC) for nucleic acid extractions, DNA genotyping, and RNA expression analysis. The project uses various technologies, including RNA sequencing and microarrays, to measure gene expression and identify expression quantitative trait loci (eQTLs). The project also includes a statistical analysis component to develop improved methods for analyzing eQTL data. The GTEx project aims to provide a comprehensive resource for the scientific community to study the relationship between genetic variation and gene expression across multiple tissues. The project includes a data release policy that ensures the rapid sharing of data while balancing the risks of identifying donors. The project also includes an ethical, legal, and social implications (ELSI) component to address the potential risks and benefits of donor recruitment and consent. The GTEx project is a large-scale initiative that aims to advance the understanding of genetic variation and gene regulation in human tissues.