The Human Urine Metabolome is a comprehensive database of 2651 confirmed human urine metabolites, their structures, concentrations, and disease associations. The study used multiple metabolomics platforms, including NMR, GC-MS, DFI/LC-MS/MS, ICP-MS, and HPLC, to identify and quantify 445 and 378 unique urine metabolites, respectively. These platforms identified a total of 209, 179, 127, 40, and 10 metabolites, respectively. The database also includes 2206 urinary compounds identified through a literature review. The Urine Metabolome Database (UMDB) is freely available and provides detailed information on each metabolite, including their structures, concentrations, and disease associations. The database also includes metabolic pathway maps and allows users to search for metabolites using various methods, including text queries, chemical formulas, and sequence searches. The UMDB contains information on 2651 detectable metabolites and 3832 concentration ranges associated with 220 different conditions. The study highlights the importance of urine as a diagnostic biofluid and the value of comprehensive metabolomic analysis in understanding human metabolism. The database is a valuable resource for researchers studying urine metabolomics and related diseases.The Human Urine Metabolome is a comprehensive database of 2651 confirmed human urine metabolites, their structures, concentrations, and disease associations. The study used multiple metabolomics platforms, including NMR, GC-MS, DFI/LC-MS/MS, ICP-MS, and HPLC, to identify and quantify 445 and 378 unique urine metabolites, respectively. These platforms identified a total of 209, 179, 127, 40, and 10 metabolites, respectively. The database also includes 2206 urinary compounds identified through a literature review. The Urine Metabolome Database (UMDB) is freely available and provides detailed information on each metabolite, including their structures, concentrations, and disease associations. The database also includes metabolic pathway maps and allows users to search for metabolites using various methods, including text queries, chemical formulas, and sequence searches. The UMDB contains information on 2651 detectable metabolites and 3832 concentration ranges associated with 220 different conditions. The study highlights the importance of urine as a diagnostic biofluid and the value of comprehensive metabolomic analysis in understanding human metabolism. The database is a valuable resource for researchers studying urine metabolomics and related diseases.