The Multidimensional Assessment of Interoceptive Awareness (MAIA) is a self-report measure developed to assess interoceptive body awareness. The development involved a systematic mixed-methods process, including literature review, conceptual framework development, evaluation of existing instruments, item development, and focus group analysis. Items were refined through cognitive testing and field testing with students and instructors of mind-body approaches. Final items were selected through an iterative process using multiple validation methods, including exploratory and confirmatory factor analyses, known groups comparison, and correlations with established measures. The resulting 32-item instrument assesses eight concepts, including body sensation awareness, emotional reaction to sensations, attention regulation, trusting body sensations, and mind-body integration. The psychometric properties of the final scales suggest that MAIA may serve as a starting point for research and further refinement. The study aimed to develop a reliable and valid measure of interoceptive awareness, which is a complex construct with multiple dimensions. The final MAIA consists of eight scales, each reflecting a specific dimension of interoceptive awareness. The scales show good internal consistency and reliability, with correlations indicating independence between scales. The field test data allowed the reduction of the tested item pool from 63 items to 32 items. The MAIA is a multidimensional instrument that may be useful for research and clinical applications in mind-body therapies.The Multidimensional Assessment of Interoceptive Awareness (MAIA) is a self-report measure developed to assess interoceptive body awareness. The development involved a systematic mixed-methods process, including literature review, conceptual framework development, evaluation of existing instruments, item development, and focus group analysis. Items were refined through cognitive testing and field testing with students and instructors of mind-body approaches. Final items were selected through an iterative process using multiple validation methods, including exploratory and confirmatory factor analyses, known groups comparison, and correlations with established measures. The resulting 32-item instrument assesses eight concepts, including body sensation awareness, emotional reaction to sensations, attention regulation, trusting body sensations, and mind-body integration. The psychometric properties of the final scales suggest that MAIA may serve as a starting point for research and further refinement. The study aimed to develop a reliable and valid measure of interoceptive awareness, which is a complex construct with multiple dimensions. The final MAIA consists of eight scales, each reflecting a specific dimension of interoceptive awareness. The scales show good internal consistency and reliability, with correlations indicating independence between scales. The field test data allowed the reduction of the tested item pool from 63 items to 32 items. The MAIA is a multidimensional instrument that may be useful for research and clinical applications in mind-body therapies.