The concept of One Health, which recognizes the interconnectedness of human, animal, and environmental health, has gained prominence in recent years due to the COVID-19 pandemic and climate change. The United Nations Sustainable Development Goals (SDGs) have set targets for health, clean water, sanitation, climate action, and sustainability in ecosystems. The One Health Quadripartite, comprising the WHO, WOAH, FAO, and UNEP, focuses on six areas: laboratory services, zoonotic disease control, neglected tropical diseases, antimicrobial resistance, food safety, and environmental health. Biomedical and clinical scientists play a crucial role in applying the One Health approach to healthcare in the 21st century. Laboratory services are essential for diagnosing infectious diseases, with microbiologists contributing to the development of testing protocols. The One Health approach encourages collaboration between medical and veterinary scientists to address zoonotic diseases, such as *Leishmania* infections, by developing and optimizing various tests. Surveillance of infectious diseases is also a key focus, with countries collecting and collating data on human pathogens and implementing notification systems. Laboratory services are vital for monitoring and surveillance, but they can also be significant sources of waste, leading to efforts to reduce environmental impact. Controlling zoonotic diseases requires understanding the interactions between humans, animals, and their environment. Examples include rabies and Echinococcus multilocularis infections, where changes in wildlife populations and human activities have influenced disease dynamics. The One Health approach emphasizes the importance of global cooperation and a broader perspective to effectively control zoonotic infections. Neglected tropical diseases (NTDs) affect poorer communities and are often transmitted through poor living conditions. The One Health approach helps in understanding the epidemiology of NTDs and finding improved control methods. Chagas disease, dengue, chikungunya, and trachoma are highlighted as examples where the environment and social factors play a significant role in disease transmission and control. Antimicrobial resistance (AMR) is a serious global threat, with the misuse of antibiotics in both human medicine and agriculture contributing to its development. The One Health approach involves multidisciplinary efforts to combat AMR, including reducing antibiotic use, improving environmental management, and enhancing public awareness. Food safety is another critical area, with the presence of toxins, pollutants, or pathogens in food requiring regular testing and proper storage and transport. The One Health approach considers the interactions between animals, the environment, and human practices to ensure food safety. In conclusion, the One Health concept integrates human, animal, and environmental health to address complex health challenges. The involvement of multidisciplinary scientists and organizations is essential for effective implementation and addressing the interconnected issues of health and sustainability.The concept of One Health, which recognizes the interconnectedness of human, animal, and environmental health, has gained prominence in recent years due to the COVID-19 pandemic and climate change. The United Nations Sustainable Development Goals (SDGs) have set targets for health, clean water, sanitation, climate action, and sustainability in ecosystems. The One Health Quadripartite, comprising the WHO, WOAH, FAO, and UNEP, focuses on six areas: laboratory services, zoonotic disease control, neglected tropical diseases, antimicrobial resistance, food safety, and environmental health. Biomedical and clinical scientists play a crucial role in applying the One Health approach to healthcare in the 21st century. Laboratory services are essential for diagnosing infectious diseases, with microbiologists contributing to the development of testing protocols. The One Health approach encourages collaboration between medical and veterinary scientists to address zoonotic diseases, such as *Leishmania* infections, by developing and optimizing various tests. Surveillance of infectious diseases is also a key focus, with countries collecting and collating data on human pathogens and implementing notification systems. Laboratory services are vital for monitoring and surveillance, but they can also be significant sources of waste, leading to efforts to reduce environmental impact. Controlling zoonotic diseases requires understanding the interactions between humans, animals, and their environment. Examples include rabies and Echinococcus multilocularis infections, where changes in wildlife populations and human activities have influenced disease dynamics. The One Health approach emphasizes the importance of global cooperation and a broader perspective to effectively control zoonotic infections. Neglected tropical diseases (NTDs) affect poorer communities and are often transmitted through poor living conditions. The One Health approach helps in understanding the epidemiology of NTDs and finding improved control methods. Chagas disease, dengue, chikungunya, and trachoma are highlighted as examples where the environment and social factors play a significant role in disease transmission and control. Antimicrobial resistance (AMR) is a serious global threat, with the misuse of antibiotics in both human medicine and agriculture contributing to its development. The One Health approach involves multidisciplinary efforts to combat AMR, including reducing antibiotic use, improving environmental management, and enhancing public awareness. Food safety is another critical area, with the presence of toxins, pollutants, or pathogens in food requiring regular testing and proper storage and transport. The One Health approach considers the interactions between animals, the environment, and human practices to ensure food safety. In conclusion, the One Health concept integrates human, animal, and environmental health to address complex health challenges. The involvement of multidisciplinary scientists and organizations is essential for effective implementation and addressing the interconnected issues of health and sustainability.