The authors present a deep analysis of the human proteome in less than 1 hour using the Orbitrap Astral mass spectrometer, along with advanced sample preparation, method development, and data processing techniques. The system, equipped with a quadrupole mass filter, a high-field Orbitrap mass analyzer, and an asymmetric track lossless (Astral) mass analyzer, offers high tandem mass spectrometry acquisition speeds of 200 Hz and detects hundreds of peptide sequences per second. Over a 30-minute active chromatographic method, the Q-Orbitrap-Astral hybrid MS collects an average of 4,319 MS scans and 438,062 tandem mass spectrometry scans, producing 235,916 peptide sequences with a 1% false discovery rate. On average, each 30-minute analysis achieves the detection of 10,411 protein groups with a 1% false discovery rate. The authors conclude that the 1-hour human proteome analysis is now within reach, highlighting the significant advancements in proteomics technology and its potential to elevate biological investigations in the future.The authors present a deep analysis of the human proteome in less than 1 hour using the Orbitrap Astral mass spectrometer, along with advanced sample preparation, method development, and data processing techniques. The system, equipped with a quadrupole mass filter, a high-field Orbitrap mass analyzer, and an asymmetric track lossless (Astral) mass analyzer, offers high tandem mass spectrometry acquisition speeds of 200 Hz and detects hundreds of peptide sequences per second. Over a 30-minute active chromatographic method, the Q-Orbitrap-Astral hybrid MS collects an average of 4,319 MS scans and 438,062 tandem mass spectrometry scans, producing 235,916 peptide sequences with a 1% false discovery rate. On average, each 30-minute analysis achieves the detection of 10,411 protein groups with a 1% false discovery rate. The authors conclude that the 1-hour human proteome analysis is now within reach, highlighting the significant advancements in proteomics technology and its potential to elevate biological investigations in the future.