The PRISMA 2020 statement is an updated guideline for reporting systematic reviews, replacing the 2009 version. It reflects advances in systematic review methods, including identifying, selecting, appraising, and synthesizing studies. The 27-item checklist, along with an expanded checklist, abstract checklist, and revised flow diagrams, aims to improve transparency and completeness in reporting. The guideline is applicable to reviews of health interventions, as well as other interventions and non-interventional studies. It includes new items on assessing certainty in evidence, declaring competing interests, and indicating publicly available data. The PRISMA 2020 statement is intended for use in both original and updated systematic reviews, with additional considerations for living reviews. It is not intended to guide the conduct of systematic reviews but to ensure all recommended information is captured. The guideline is accompanied by an explanation and elaboration paper, a web-based checklist tool, and templates for flow diagrams. The PRISMA 2020 statement is designed to enhance the quality and reliability of systematic reviews, facilitating evidence-based decision-making. It is endorsed by numerous journals and organizations and is available in multiple languages. The guideline is supported by a range of resources, including online tools and templates, and is intended to be used by authors, editors, and peer reviewers to ensure comprehensive and accurate reporting of systematic reviews.The PRISMA 2020 statement is an updated guideline for reporting systematic reviews, replacing the 2009 version. It reflects advances in systematic review methods, including identifying, selecting, appraising, and synthesizing studies. The 27-item checklist, along with an expanded checklist, abstract checklist, and revised flow diagrams, aims to improve transparency and completeness in reporting. The guideline is applicable to reviews of health interventions, as well as other interventions and non-interventional studies. It includes new items on assessing certainty in evidence, declaring competing interests, and indicating publicly available data. The PRISMA 2020 statement is intended for use in both original and updated systematic reviews, with additional considerations for living reviews. It is not intended to guide the conduct of systematic reviews but to ensure all recommended information is captured. The guideline is accompanied by an explanation and elaboration paper, a web-based checklist tool, and templates for flow diagrams. The PRISMA 2020 statement is designed to enhance the quality and reliability of systematic reviews, facilitating evidence-based decision-making. It is endorsed by numerous journals and organizations and is available in multiple languages. The guideline is supported by a range of resources, including online tools and templates, and is intended to be used by authors, editors, and peer reviewers to ensure comprehensive and accurate reporting of systematic reviews.