Mesenchymal stem cells (MSCs) have shown great potential in promoting skin healing and regeneration. MSCs can differentiate into various cell types involved in wound healing, such as dermal fibroblasts, endothelial cells, and keratinocytes. They also promote neovascularization, cellular regeneration, and tissue healing through paracrine and autocrine signaling. MSCs have been extensively studied in the context of burn healing and chronic wound repair. Recent preclinical and clinical studies have demonstrated their therapeutic effects in wound healing and skin regeneration. Strategies to enhance MSC therapy include combining them with tissue engineering techniques, such as biodegradable scaffolds and hydrogels, to improve skin repair capacity. Additionally, MSCs' paracrine and autocrine characteristics offer potential for cell-free therapies in stem cell-based treatments. MSCs have shown promise in treating chronic refractory wounds and burn injuries by reducing inflammation, promoting angiogenesis, and enhancing tissue repair. They also have potential in skin rejuvenation and anti-aging by reducing oxidative stress and promoting collagen synthesis. MSCs can be delivered to wounds using various methods, including hydrogels, scaffolds, and cell sheets, which improve their retention and therapeutic effects. Conditioned media from MSCs can also promote wound healing by accelerating epithelialization and reducing inflammation. MSCs combined with other techniques, such as 3D bioprinting, can further enhance skin repair and regeneration. Overall, MSCs offer a promising approach for skin repair and regeneration with potential for future clinical applications.Mesenchymal stem cells (MSCs) have shown great potential in promoting skin healing and regeneration. MSCs can differentiate into various cell types involved in wound healing, such as dermal fibroblasts, endothelial cells, and keratinocytes. They also promote neovascularization, cellular regeneration, and tissue healing through paracrine and autocrine signaling. MSCs have been extensively studied in the context of burn healing and chronic wound repair. Recent preclinical and clinical studies have demonstrated their therapeutic effects in wound healing and skin regeneration. Strategies to enhance MSC therapy include combining them with tissue engineering techniques, such as biodegradable scaffolds and hydrogels, to improve skin repair capacity. Additionally, MSCs' paracrine and autocrine characteristics offer potential for cell-free therapies in stem cell-based treatments. MSCs have shown promise in treating chronic refractory wounds and burn injuries by reducing inflammation, promoting angiogenesis, and enhancing tissue repair. They also have potential in skin rejuvenation and anti-aging by reducing oxidative stress and promoting collagen synthesis. MSCs can be delivered to wounds using various methods, including hydrogels, scaffolds, and cell sheets, which improve their retention and therapeutic effects. Conditioned media from MSCs can also promote wound healing by accelerating epithelialization and reducing inflammation. MSCs combined with other techniques, such as 3D bioprinting, can further enhance skin repair and regeneration. Overall, MSCs offer a promising approach for skin repair and regeneration with potential for future clinical applications.