This study investigates the role of sulfhydryl (thiol) groups in oral and periodontal diseases. Sulfhydryl groups are crucial for neutralizing foreign organic compounds and reducing peroxides. The research assesses the concentration of sulfhydryl groups in gingival tissue from healthy individuals and those with gingivitis or periodontitis using spectrophotometric analysis with dithionitrobenzoate (DTNB). The sample is divided into three groups: periodontal health, gingivitis, and periodontitis. Statistical analysis reveals significantly lower sulfhydryl group concentrations in gingivitis and periodontitis patients compared to healthy individuals. These findings highlight the importance of sulfhydryl groups in defending against free radicals, detoxification, signal transduction, and apoptosis. The study suggests that restoring or enhancing sulfhydryl group levels could be a therapeutic approach to manage and ameliorate periodontal diseases.This study investigates the role of sulfhydryl (thiol) groups in oral and periodontal diseases. Sulfhydryl groups are crucial for neutralizing foreign organic compounds and reducing peroxides. The research assesses the concentration of sulfhydryl groups in gingival tissue from healthy individuals and those with gingivitis or periodontitis using spectrophotometric analysis with dithionitrobenzoate (DTNB). The sample is divided into three groups: periodontal health, gingivitis, and periodontitis. Statistical analysis reveals significantly lower sulfhydryl group concentrations in gingivitis and periodontitis patients compared to healthy individuals. These findings highlight the importance of sulfhydryl groups in defending against free radicals, detoxification, signal transduction, and apoptosis. The study suggests that restoring or enhancing sulfhydryl group levels could be a therapeutic approach to manage and ameliorate periodontal diseases.