This study presents the results of a global survey of 65,819 individuals across 67 countries, assessing attitudes towards vaccination. The survey explores perceptions of vaccine importance, safety, effectiveness, and religious compatibility. The findings reveal that overall sentiment towards vaccination is positive across all regions, but there is significant variation between countries. Vaccine safety concerns are particularly high in the European region, with 41% of respondents in France and 36% in Bosnia & Herzegovina disagreeing that vaccines are safe, compared to a global average of 13%. Older age groups and Roman Catholics tend to have more positive views on vaccines, while the Western Pacific region shows the highest level of religious incompatibility with vaccines. Countries with higher levels of education and access to health services are associated with lower positive vaccine sentiment, suggesting an inverse relationship between vaccine attitudes and socio-economic status. The study uses hierarchical regression models to examine the relationship between individual and country-level socio-economic factors and vaccine attitudes. Results show that males are less likely to think vaccines are important than females, but there are no significant differences in vaccine safety, effectiveness, or religious compatibility between sexes. Older individuals are more likely to believe vaccines are effective and to express religious incompatibilities. Higher levels of education are associated with more positive views on vaccine importance, effectiveness, and religious compatibility, but not on vaccine safety. Countries with higher levels of education and skilled health staff are associated with less positive vaccine sentiment. The study highlights the importance of monitoring vaccine confidence and immunization rates at the national and sub-national levels to identify populations with declining confidence. These populations should be prioritized for further investigation into the drivers of negative sentiment and to inform appropriate interventions to prevent adverse public health outcomes. The findings suggest that vaccine confidence is influenced by a range of factors, including socio-economic status, education, and religious beliefs. The study also emphasizes the need for continued global monitoring of vaccine confidence to inform public health policy and interventions.This study presents the results of a global survey of 65,819 individuals across 67 countries, assessing attitudes towards vaccination. The survey explores perceptions of vaccine importance, safety, effectiveness, and religious compatibility. The findings reveal that overall sentiment towards vaccination is positive across all regions, but there is significant variation between countries. Vaccine safety concerns are particularly high in the European region, with 41% of respondents in France and 36% in Bosnia & Herzegovina disagreeing that vaccines are safe, compared to a global average of 13%. Older age groups and Roman Catholics tend to have more positive views on vaccines, while the Western Pacific region shows the highest level of religious incompatibility with vaccines. Countries with higher levels of education and access to health services are associated with lower positive vaccine sentiment, suggesting an inverse relationship between vaccine attitudes and socio-economic status. The study uses hierarchical regression models to examine the relationship between individual and country-level socio-economic factors and vaccine attitudes. Results show that males are less likely to think vaccines are important than females, but there are no significant differences in vaccine safety, effectiveness, or religious compatibility between sexes. Older individuals are more likely to believe vaccines are effective and to express religious incompatibilities. Higher levels of education are associated with more positive views on vaccine importance, effectiveness, and religious compatibility, but not on vaccine safety. Countries with higher levels of education and skilled health staff are associated with less positive vaccine sentiment. The study highlights the importance of monitoring vaccine confidence and immunization rates at the national and sub-national levels to identify populations with declining confidence. These populations should be prioritized for further investigation into the drivers of negative sentiment and to inform appropriate interventions to prevent adverse public health outcomes. The findings suggest that vaccine confidence is influenced by a range of factors, including socio-economic status, education, and religious beliefs. The study also emphasizes the need for continued global monitoring of vaccine confidence to inform public health policy and interventions.