Parents of premature infants in the NICU experience high levels of stress, with 73.6% reporting high overall stress. Stress was most prevalent in domains such as sight and sound (80.3%), infants' appearance (69%), and parent-infant relationship (81.4%). Key factors contributing to stress included infants on mechanical ventilators, previous neonatal death, living far from the hospital, cesarean delivery, low income, and prolonged hospitalization. Mothers were more likely to experience high stress than fathers. Protective factors included breastfeeding, daily visits, and having living children. The study highlights the need for interventions to support parents, including education, counseling, and emotional support for both parents and healthcare staff. Further research is needed to explore additional factors and develop comprehensive strategies to help parents manage stress effectively.Parents of premature infants in the NICU experience high levels of stress, with 73.6% reporting high overall stress. Stress was most prevalent in domains such as sight and sound (80.3%), infants' appearance (69%), and parent-infant relationship (81.4%). Key factors contributing to stress included infants on mechanical ventilators, previous neonatal death, living far from the hospital, cesarean delivery, low income, and prolonged hospitalization. Mothers were more likely to experience high stress than fathers. Protective factors included breastfeeding, daily visits, and having living children. The study highlights the need for interventions to support parents, including education, counseling, and emotional support for both parents and healthcare staff. Further research is needed to explore additional factors and develop comprehensive strategies to help parents manage stress effectively.