The University of California Santa Cruz (UCSC) Genome Browser Database (GBD) has been updated to include additional species and enhancements to its tools and data. The GBD now supports about a dozen vertebrate species and several major model organisms, with a focus on human and mouse genomes. New features include multi-species alignments and conservation scores, improved gene annotations, expression data, variation data, and visualization tools such as 'wiggle' tracks for continuous-valued data. The Table Browser has been redesigned to support complex queries and relational queries between tables. New tools like the Gene Sorter and In Silico PCR utility have been added for gene-focused queries and primer pair searches. The database continues to emphasize comparative analysis and conservation measures, with ongoing improvements in user session management and data management. The UCSC GBD is a valuable resource for researchers and the scientific community, providing comprehensive and interactive access to genomic data.The University of California Santa Cruz (UCSC) Genome Browser Database (GBD) has been updated to include additional species and enhancements to its tools and data. The GBD now supports about a dozen vertebrate species and several major model organisms, with a focus on human and mouse genomes. New features include multi-species alignments and conservation scores, improved gene annotations, expression data, variation data, and visualization tools such as 'wiggle' tracks for continuous-valued data. The Table Browser has been redesigned to support complex queries and relational queries between tables. New tools like the Gene Sorter and In Silico PCR utility have been added for gene-focused queries and primer pair searches. The database continues to emphasize comparative analysis and conservation measures, with ongoing improvements in user session management and data management. The UCSC GBD is a valuable resource for researchers and the scientific community, providing comprehensive and interactive access to genomic data.