The Vienna RNA Websuite is a comprehensive collection of tools for RNA sequence folding, design, and analysis, providing a web interface to the Vienna RNA package. It includes programs for folding single and aligned sequences, predicting RNA-RNA interactions, and designing sequences with specific structures. The suite also offers analysis of folding landscapes using the barriers program and structural RNA alignments using LocARNA. The web server and software packages are freely accessible at http://rna.tbi.univie.ac.at/. The tools are designed to provide a uniform interface, with input fields, parameter settings, and detailed output, including graphical representations. The servers support various input formats and offer interactive features such as email notifications and SVG images. The article details the functionalities of each server, including RNAfold for secondary structure prediction, RNAalifold for consensus structure prediction, RNAinverse for sequence design, RNAcofold and RNAup for RNA-RNA interaction prediction, barriers for folding kinetics analysis, and LocARNA for structural alignment. The implementation uses Apache, Perl, BioPerl, CGI, and JavaScript, and the servers are powered by Intel Core 2 Quad machines.The Vienna RNA Websuite is a comprehensive collection of tools for RNA sequence folding, design, and analysis, providing a web interface to the Vienna RNA package. It includes programs for folding single and aligned sequences, predicting RNA-RNA interactions, and designing sequences with specific structures. The suite also offers analysis of folding landscapes using the barriers program and structural RNA alignments using LocARNA. The web server and software packages are freely accessible at http://rna.tbi.univie.ac.at/. The tools are designed to provide a uniform interface, with input fields, parameter settings, and detailed output, including graphical representations. The servers support various input formats and offer interactive features such as email notifications and SVG images. The article details the functionalities of each server, including RNAfold for secondary structure prediction, RNAalifold for consensus structure prediction, RNAinverse for sequence design, RNAcofold and RNAup for RNA-RNA interaction prediction, barriers for folding kinetics analysis, and LocARNA for structural alignment. The implementation uses Apache, Perl, BioPerl, CGI, and JavaScript, and the servers are powered by Intel Core 2 Quad machines.