The article discusses the art of finding the right drug target, focusing on emerging methods and strategies. Drug targets are specific molecules in biological tissues and body fluids that interact with drugs. Drug target discovery is a key component of drug discovery and is essential for the development of new drugs in areas such as cancer therapy and precision medicine. Traditional in vitro or in vivo target discovery methods are time-consuming and labor-intensive, limiting the pace of drug discovery. With the development of modern discovery methods, the discovery and application of various emerging technologies have greatly improved the efficiency of drug discovery, shortened the cycle time and reduced the cost. This review provides a comprehensive overview of various emerging drug target discovery strategies, including computer-assisted approaches, drug affinity response target stability, multiomics analysis, gene editing, and NMD, and discusses the effectiveness and limitations of the various approaches, as well as their application in real cases. Through the review of the above related contents, a general overview of the development of novel drug targets and disease treatment strategies will be provided, and a theoretical basis will be provided for those who are engaged in pharmaceutical science research. The article also discusses the emergence of new drug target discovery methods, including drug-centred and disease-centred target discovery methods. It highlights the advantages and limitations of these methods, as well as their applications in the discovery of drug targets for the treatment of human diseases. The article also discusses the application of multiomics approaches in drug target discovery, which involves the integration of various omics data to identify potential drug targets. The article concludes that the discovery of drug targets is a critical step in the drug discovery process, and the combined application of multiple methods is more common in actual cases of target discovery. The combined application of multiple target discovery methods has more obvious advantages, such as gaining a more comprehensive understanding of the diseases' occurrence process, influencing mechanisms, and expression patterns of related genes. This will facilitate subsequent in-depth research and analysis of drug targets. The article also discusses the challenges faced in the drug target discovery process, such as data processing and experimental design. Overall, the article provides a comprehensive overview of the current state of drug target discovery methods and strategies, highlighting the importance of emerging methods in the development of new drugs.The article discusses the art of finding the right drug target, focusing on emerging methods and strategies. Drug targets are specific molecules in biological tissues and body fluids that interact with drugs. Drug target discovery is a key component of drug discovery and is essential for the development of new drugs in areas such as cancer therapy and precision medicine. Traditional in vitro or in vivo target discovery methods are time-consuming and labor-intensive, limiting the pace of drug discovery. With the development of modern discovery methods, the discovery and application of various emerging technologies have greatly improved the efficiency of drug discovery, shortened the cycle time and reduced the cost. This review provides a comprehensive overview of various emerging drug target discovery strategies, including computer-assisted approaches, drug affinity response target stability, multiomics analysis, gene editing, and NMD, and discusses the effectiveness and limitations of the various approaches, as well as their application in real cases. Through the review of the above related contents, a general overview of the development of novel drug targets and disease treatment strategies will be provided, and a theoretical basis will be provided for those who are engaged in pharmaceutical science research. The article also discusses the emergence of new drug target discovery methods, including drug-centred and disease-centred target discovery methods. It highlights the advantages and limitations of these methods, as well as their applications in the discovery of drug targets for the treatment of human diseases. The article also discusses the application of multiomics approaches in drug target discovery, which involves the integration of various omics data to identify potential drug targets. The article concludes that the discovery of drug targets is a critical step in the drug discovery process, and the combined application of multiple methods is more common in actual cases of target discovery. The combined application of multiple target discovery methods has more obvious advantages, such as gaining a more comprehensive understanding of the diseases' occurrence process, influencing mechanisms, and expression patterns of related genes. This will facilitate subsequent in-depth research and analysis of drug targets. The article also discusses the challenges faced in the drug target discovery process, such as data processing and experimental design. Overall, the article provides a comprehensive overview of the current state of drug target discovery methods and strategies, highlighting the importance of emerging methods in the development of new drugs.