The Chloroflexota phylum, previously known as green non-sulfur bacteria, consists of diverse microorganisms found in various environments, including terrestrial and aquatic ones. This phylum is divided into eight classes, each with distinct metabolic strategies, enabling them to thrive in extreme environments. The metabolic diversity of Chloroflexota includes anoxygenic phototrophy, obligate anaerobic heterotrophy, organohalide respiration, and facultative or aerobic heterotrophy. These bacteria are found in a wide range of environments, from natural to industrial, and their metabolic versatility allows them to adapt to various ecological niches. Chloroflexota members exhibit a variety of metabolic capabilities, such as the ability to fix inorganic carbon using the 3-hydroxypropionate bi-cycle, perform denitrification, and utilize toxic compounds as electron acceptors. These metabolic strategies are driven by the interplay between temperature, oxygen availability, and energy metabolism, enabling the phylum to colonize a wide range of ecological niches. Specific members of the phylum, such as Chloroflexus aurantiacus, showcase thermophilic and phototrophic growth, while members of the Anaerolineae class are known for their role in the degradation of complex organic compounds, contributing significantly to the carbon cycle in anaerobic environments. The biotechnological potential of Chloroflexota is significant, with applications in the production of enzymes, energy, and value-added substances. For example, Chloroflexus aurantiacus has been used to produce enzymes such as α-L-rhamnosidase and alcohol dehydrogenase, which have applications in various industries. Additionally, Chloroflexota bacteria can be used in wastewater treatment, soil treatment, and biodegradation technologies, contributing to the removal of pollutants and the degradation of contaminants. The metabolic diversity of Chloroflexota also includes the ability to perform organohalide respiration, which is important in bioremediation applications. Dehalococcoides, a class within the Chloroflexota phylum, is known for its ability to dechlorinate chlorinated and brominated alkanes under strict anaerobic conditions, making them valuable in the bioremediation of contaminated sites. Furthermore, the ability of Chloroflexota bacteria to fix carbon and produce biodegradable organic matter makes them important in soil treatment and composting processes. Overall, the metabolic diversity and adaptability of Chloroflexota bacteria make them valuable for various biotechnological applications, including environmental remediation, energy production, and the production of value-added substances. The unique characteristics of these bacteria, such as their ability to thrive in extreme environments and their metabolic versatility, highlight their potential for use in a wide range of biotechnological processes.The Chloroflexota phylum, previously known as green non-sulfur bacteria, consists of diverse microorganisms found in various environments, including terrestrial and aquatic ones. This phylum is divided into eight classes, each with distinct metabolic strategies, enabling them to thrive in extreme environments. The metabolic diversity of Chloroflexota includes anoxygenic phototrophy, obligate anaerobic heterotrophy, organohalide respiration, and facultative or aerobic heterotrophy. These bacteria are found in a wide range of environments, from natural to industrial, and their metabolic versatility allows them to adapt to various ecological niches. Chloroflexota members exhibit a variety of metabolic capabilities, such as the ability to fix inorganic carbon using the 3-hydroxypropionate bi-cycle, perform denitrification, and utilize toxic compounds as electron acceptors. These metabolic strategies are driven by the interplay between temperature, oxygen availability, and energy metabolism, enabling the phylum to colonize a wide range of ecological niches. Specific members of the phylum, such as Chloroflexus aurantiacus, showcase thermophilic and phototrophic growth, while members of the Anaerolineae class are known for their role in the degradation of complex organic compounds, contributing significantly to the carbon cycle in anaerobic environments. The biotechnological potential of Chloroflexota is significant, with applications in the production of enzymes, energy, and value-added substances. For example, Chloroflexus aurantiacus has been used to produce enzymes such as α-L-rhamnosidase and alcohol dehydrogenase, which have applications in various industries. Additionally, Chloroflexota bacteria can be used in wastewater treatment, soil treatment, and biodegradation technologies, contributing to the removal of pollutants and the degradation of contaminants. The metabolic diversity of Chloroflexota also includes the ability to perform organohalide respiration, which is important in bioremediation applications. Dehalococcoides, a class within the Chloroflexota phylum, is known for its ability to dechlorinate chlorinated and brominated alkanes under strict anaerobic conditions, making them valuable in the bioremediation of contaminated sites. Furthermore, the ability of Chloroflexota bacteria to fix carbon and produce biodegradable organic matter makes them important in soil treatment and composting processes. Overall, the metabolic diversity and adaptability of Chloroflexota bacteria make them valuable for various biotechnological applications, including environmental remediation, energy production, and the production of value-added substances. The unique characteristics of these bacteria, such as their ability to thrive in extreme environments and their metabolic versatility, highlight their potential for use in a wide range of biotechnological processes.