The Carbohydrate-Active Enzymes database (CAZy) provides a comprehensive and continuously updated resource for the classification, sequence, structure, and functional information of CAZymes (carbohydrate-active enzymes). The database, accessible at http://www.cazy.org, has seen significant expansion and improvement over the past five years. Key changes include a redesign of the website interface, the addition of new sections and links, and enhanced interactivity. The database now reports sequence information on nearly 340,000 CAZymes, with a notable increase in the number of biochemically characterized and structurally resolved enzymes. The classification scheme has been expanded to include new families and subfamilies, and the database now supports genome analysis, providing insights into the CAZymes encoded by various organisms. Additionally, the database now includes detailed structural information, such as resolution and carbohydrate ligands, for over 1,400 CAZymes. Future directions focus on improving the reliability of substrate prediction and expanding the scope of subfamily definitions.The Carbohydrate-Active Enzymes database (CAZy) provides a comprehensive and continuously updated resource for the classification, sequence, structure, and functional information of CAZymes (carbohydrate-active enzymes). The database, accessible at http://www.cazy.org, has seen significant expansion and improvement over the past five years. Key changes include a redesign of the website interface, the addition of new sections and links, and enhanced interactivity. The database now reports sequence information on nearly 340,000 CAZymes, with a notable increase in the number of biochemically characterized and structurally resolved enzymes. The classification scheme has been expanded to include new families and subfamilies, and the database now supports genome analysis, providing insights into the CAZymes encoded by various organisms. Additionally, the database now includes detailed structural information, such as resolution and carbohydrate ligands, for over 1,400 CAZymes. Future directions focus on improving the reliability of substrate prediction and expanding the scope of subfamily definitions.