The article discusses the concept of lipid rafts, which are relatively ordered membrane domains in cell membranes, formed by the preferential association of cholesterol and saturated lipids. These domains are thought to play a role in various cellular functions, including signal transduction, protein trafficking, and cell adhesion. The article reviews the history of the lipid raft hypothesis, the methods used to study lipid rafts, and the current understanding of their composition, regulation, and physiological relevance. It also discusses the challenges in directly observing lipid rafts in live cells and the potential of new technologies, such as super-resolution optical microscopy, to overcome these challenges. The article highlights the importance of lipid rafts in immune signaling, host-pathogen interactions, cancer, and cardiovascular diseases. It also discusses the role of various proteins and lipids in the formation and function of lipid rafts, as well as the potential of lipid raft-targeting drugs in therapeutic applications. The article concludes with a discussion of the future directions in lipid raft research, including the need for more precise methods to study these domains and their role in cellular processes.The article discusses the concept of lipid rafts, which are relatively ordered membrane domains in cell membranes, formed by the preferential association of cholesterol and saturated lipids. These domains are thought to play a role in various cellular functions, including signal transduction, protein trafficking, and cell adhesion. The article reviews the history of the lipid raft hypothesis, the methods used to study lipid rafts, and the current understanding of their composition, regulation, and physiological relevance. It also discusses the challenges in directly observing lipid rafts in live cells and the potential of new technologies, such as super-resolution optical microscopy, to overcome these challenges. The article highlights the importance of lipid rafts in immune signaling, host-pathogen interactions, cancer, and cardiovascular diseases. It also discusses the role of various proteins and lipids in the formation and function of lipid rafts, as well as the potential of lipid raft-targeting drugs in therapeutic applications. The article concludes with a discussion of the future directions in lipid raft research, including the need for more precise methods to study these domains and their role in cellular processes.