Squalene, a natural triterpenoid unsaturated hydrocarbon found in shark liver and plant seeds, has various physiological functions including anti-inflammatory and antioxidant properties. This review discusses the physiological functions of squalene and its potential applications in animal husbandry. Squalene plays a role in reducing oxidative stress, improving cardiovascular health, enhancing gut health, and protecting skin. It also has potential as a vaccine adjuvant, antibacterial agent, and sustained-release carrier. Squalene can alleviate stress in livestock and poultry by reducing oxidative damage and improving antioxidant capacity. It supports gut health by enhancing antioxidant enzyme activity and reducing inflammation. Squalene also protects skin health by providing antioxidant and moisturizing properties. Additionally, it has detoxifying effects, helping to remove toxins and reduce their toxicity. Squalene can improve the quality of livestock products by enhancing meat quality and reproductive performance. The application of squalene in animal production shows great potential, with further research needed to explore its optimal use in different stages of animal growth. Squalene's ability to reduce inflammation, oxidative stress, and improve antioxidant capacity makes it a valuable component in animal husbandry. Its potential as a feed additive, vaccine adjuvant, and drug delivery system highlights its importance in promoting green and healthy farming practices.Squalene, a natural triterpenoid unsaturated hydrocarbon found in shark liver and plant seeds, has various physiological functions including anti-inflammatory and antioxidant properties. This review discusses the physiological functions of squalene and its potential applications in animal husbandry. Squalene plays a role in reducing oxidative stress, improving cardiovascular health, enhancing gut health, and protecting skin. It also has potential as a vaccine adjuvant, antibacterial agent, and sustained-release carrier. Squalene can alleviate stress in livestock and poultry by reducing oxidative damage and improving antioxidant capacity. It supports gut health by enhancing antioxidant enzyme activity and reducing inflammation. Squalene also protects skin health by providing antioxidant and moisturizing properties. Additionally, it has detoxifying effects, helping to remove toxins and reduce their toxicity. Squalene can improve the quality of livestock products by enhancing meat quality and reproductive performance. The application of squalene in animal production shows great potential, with further research needed to explore its optimal use in different stages of animal growth. Squalene's ability to reduce inflammation, oxidative stress, and improve antioxidant capacity makes it a valuable component in animal husbandry. Its potential as a feed additive, vaccine adjuvant, and drug delivery system highlights its importance in promoting green and healthy farming practices.