The tomato genome sequence provides insights into the evolution of fleshy fruits. The Tomato Genome Consortium sequenced the genome of the inbred tomato cultivar 'Heinz 1706' and its wild relative, Solanum pimpinellifolium, and compared them to the potato genome. The two tomato genomes show only 0.6% nucleotide divergence and signs of recent admixture, but show more than 8% divergence from potato, with nine large and several smaller inversions. The Solanum lineage has experienced two consecutive genome triplications, which set the stage for the neofunctionalization of genes controlling fruit characteristics. The genome of the inbred tomato cultivar 'Heinz 1706' was sequenced and assembled using a combination of Sanger and next generation technologies. The predicted genome size is approximately 900 Mb, with 760 Mb assembled in 91 scaffolds aligned to the 12 tomato chromosomes. The genome of S. pimpinellifolium was sequenced and assembled de novo using Illumina short reads, yielding a 739 Mb draft genome. The genome of tomato consists of pericentric heterochromatin and distal euchromatin, with repeats concentrated within and around centromeres. The genome is highly syntenic with other economically important Solanaceae. The genome sequences of tomato, S. pimpinellifolium, and potato provide a starting point for comparing gene family evolution and sub-functionalization in the Solanaceae. The genome sequences also provide a basis for understanding the bottlenecks that have narrowed tomato genetic diversity. The Tomato Genome Consortium includes researchers from various institutions around the world. The study was supported by multiple funding sources. The authors declare no competing financial interests. The paper is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike licence.The tomato genome sequence provides insights into the evolution of fleshy fruits. The Tomato Genome Consortium sequenced the genome of the inbred tomato cultivar 'Heinz 1706' and its wild relative, Solanum pimpinellifolium, and compared them to the potato genome. The two tomato genomes show only 0.6% nucleotide divergence and signs of recent admixture, but show more than 8% divergence from potato, with nine large and several smaller inversions. The Solanum lineage has experienced two consecutive genome triplications, which set the stage for the neofunctionalization of genes controlling fruit characteristics. The genome of the inbred tomato cultivar 'Heinz 1706' was sequenced and assembled using a combination of Sanger and next generation technologies. The predicted genome size is approximately 900 Mb, with 760 Mb assembled in 91 scaffolds aligned to the 12 tomato chromosomes. The genome of S. pimpinellifolium was sequenced and assembled de novo using Illumina short reads, yielding a 739 Mb draft genome. The genome of tomato consists of pericentric heterochromatin and distal euchromatin, with repeats concentrated within and around centromeres. The genome is highly syntenic with other economically important Solanaceae. The genome sequences of tomato, S. pimpinellifolium, and potato provide a starting point for comparing gene family evolution and sub-functionalization in the Solanaceae. The genome sequences also provide a basis for understanding the bottlenecks that have narrowed tomato genetic diversity. The Tomato Genome Consortium includes researchers from various institutions around the world. The study was supported by multiple funding sources. The authors declare no competing financial interests. The paper is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike licence.