This study aimed to investigate the relationship between serum total chemerin and CMKLR1 activation in obese and normal-weight humans. Blood samples were collected from four obese (BMI >30) and four normal-weight (BMI 20-25) female subjects after an overnight fast and at regular intervals following a breakfast meal over a 6-hour period. A cellular CMKLR1-luminescent reporter assay and a pan-chemerin ELISA were used to measure CMKLR1 activation and total chemerin concentrations, respectively. Results showed that serum total chemerin concentration was significantly higher in obese subjects compared to normal-weight subjects (17.9 ± 1.8 vs 10.9 ± 0.5 nM, P < 0.05). However, serum activation of CMKLR1 was similar in both groups, and the CMKLR1 activation/total chemerin ratio was lower in obese subjects (0.33 ± 0.04 vs 0.58 ± 0.05, P < 0.05). After breakfast, serum total chemerin or CMKLR1 activation did not differ from baseline values. These findings suggest that increased serum total chemerin in obesity does not necessarily lead to proportionally greater CMKLR1 activation, indicating potential impaired processing or enhanced degradation of active chemerin in obese individuals.This study aimed to investigate the relationship between serum total chemerin and CMKLR1 activation in obese and normal-weight humans. Blood samples were collected from four obese (BMI >30) and four normal-weight (BMI 20-25) female subjects after an overnight fast and at regular intervals following a breakfast meal over a 6-hour period. A cellular CMKLR1-luminescent reporter assay and a pan-chemerin ELISA were used to measure CMKLR1 activation and total chemerin concentrations, respectively. Results showed that serum total chemerin concentration was significantly higher in obese subjects compared to normal-weight subjects (17.9 ± 1.8 vs 10.9 ± 0.5 nM, P < 0.05). However, serum activation of CMKLR1 was similar in both groups, and the CMKLR1 activation/total chemerin ratio was lower in obese subjects (0.33 ± 0.04 vs 0.58 ± 0.05, P < 0.05). After breakfast, serum total chemerin or CMKLR1 activation did not differ from baseline values. These findings suggest that increased serum total chemerin in obesity does not necessarily lead to proportionally greater CMKLR1 activation, indicating potential impaired processing or enhanced degradation of active chemerin in obese individuals.