Researchers have developed a method to construct complex three-dimensional (3D) structures using short synthetic DNA strands called "DNA bricks." These bricks, each 32 nucleotides long with four 8-base binding domains, self-assemble into prescribed 3D shapes through one-step annealing reactions. The method allows for the creation of 102 distinct shapes with sophisticated surface features and intricate interior cavities. The modular nature of DNA bricks enables the construction of a "molecular canvas" with dimensions of 10 × 10 × 10 voxels, where each voxel is 2.5 nm × 2.5 nm × 2.7 nm. By selecting subsets of bricks from this canvas, researchers can build a wide range of 3D structures, including solid and hollow shapes with complex geometries. The approach offers a simple, robust, and versatile framework for assembling complex 3D nanostructures using only short synthetic DNA strands. The study also demonstrates the ability to design and construct a variety of 3D shapes, including those with intricate cavities, tunnels, and geometric features. The method has potential applications in nanotechnology, including the arrangement of functional molecules, bioimaging, and drug delivery. The DNA brick approach provides a new level of geometrical sophistication and offers a generalizable framework for constructing diverse 3D structures. The study highlights the potential of DNA-based self-assembly for creating complex nanostructures with precise control over their geometry and function.Researchers have developed a method to construct complex three-dimensional (3D) structures using short synthetic DNA strands called "DNA bricks." These bricks, each 32 nucleotides long with four 8-base binding domains, self-assemble into prescribed 3D shapes through one-step annealing reactions. The method allows for the creation of 102 distinct shapes with sophisticated surface features and intricate interior cavities. The modular nature of DNA bricks enables the construction of a "molecular canvas" with dimensions of 10 × 10 × 10 voxels, where each voxel is 2.5 nm × 2.5 nm × 2.7 nm. By selecting subsets of bricks from this canvas, researchers can build a wide range of 3D structures, including solid and hollow shapes with complex geometries. The approach offers a simple, robust, and versatile framework for assembling complex 3D nanostructures using only short synthetic DNA strands. The study also demonstrates the ability to design and construct a variety of 3D shapes, including those with intricate cavities, tunnels, and geometric features. The method has potential applications in nanotechnology, including the arrangement of functional molecules, bioimaging, and drug delivery. The DNA brick approach provides a new level of geometrical sophistication and offers a generalizable framework for constructing diverse 3D structures. The study highlights the potential of DNA-based self-assembly for creating complex nanostructures with precise control over their geometry and function.