The article introduces the TimeTree resource, a public knowledge base that provides access to species divergence times derived from molecular sequence data. The resource has been significantly expanded, tripling the number of species (over 97,000) and studies (over 3,000). New features include timetrees of groups of species and timelines tracing the evolution of a species through time, integrated with Earth and environmental history. The resource is designed to facilitate data-driven retrieval of peer-reviewed publications and divergence time estimates relevant to user queries. The next-generation TimeTree resource is publicly available online at http://www.timetree.org, offering advanced functionalities such as custom timetrees and timelines, and context-sensitive information. The article also details the multi-tiered web architecture of TimeTree, including its computational database, data management, and interactive exploration capabilities.The article introduces the TimeTree resource, a public knowledge base that provides access to species divergence times derived from molecular sequence data. The resource has been significantly expanded, tripling the number of species (over 97,000) and studies (over 3,000). New features include timetrees of groups of species and timelines tracing the evolution of a species through time, integrated with Earth and environmental history. The resource is designed to facilitate data-driven retrieval of peer-reviewed publications and divergence time estimates relevant to user queries. The next-generation TimeTree resource is publicly available online at http://www.timetree.org, offering advanced functionalities such as custom timetrees and timelines, and context-sensitive information. The article also details the multi-tiered web architecture of TimeTree, including its computational database, data management, and interactive exploration capabilities.