The study by P. George and D. H. Irvine investigates the pH-dependent ionization of the haem-linked group in MetMb, leading to the conclusion that 2 moles of H+ are liberated per mole of acidic MetMb. The results support the ferryl ion structure for the higher oxidation state of myoglobin, which is provisionally named ferrylmyoglobin. The study also examines the temperature dependence of $K_{\text{obs}}$, ionic strength dependence, and the distribution patterns of various enzymes in rat liver tissue, revealing distinct patterns for different enzymes and suggesting the existence of multiple classes of cytoplasmic granules. The findings confirm the presence of mitochondria, microsomes, and a special group of granules containing acid phosphatase, and propose the existence of lysosomes based on the distribution of hydrolases. The study highlights the importance of these granules in cellular oxidations and intracellular digestion.The study by P. George and D. H. Irvine investigates the pH-dependent ionization of the haem-linked group in MetMb, leading to the conclusion that 2 moles of H+ are liberated per mole of acidic MetMb. The results support the ferryl ion structure for the higher oxidation state of myoglobin, which is provisionally named ferrylmyoglobin. The study also examines the temperature dependence of $K_{\text{obs}}$, ionic strength dependence, and the distribution patterns of various enzymes in rat liver tissue, revealing distinct patterns for different enzymes and suggesting the existence of multiple classes of cytoplasmic granules. The findings confirm the presence of mitochondria, microsomes, and a special group of granules containing acid phosphatase, and propose the existence of lysosomes based on the distribution of hydrolases. The study highlights the importance of these granules in cellular oxidations and intracellular digestion.