This volume of "Topics in Fluorescence Spectroscopy" focuses on the biochemical applications of fluorescence spectroscopy. Edited by Joseph R. Lakowicz, it is the third in a series of three volumes that cover techniques, principles, and applications in fluorescence spectroscopy. The book highlights recent advancements in time-resolution, data analysis, and instrumentation, which have enabled more detailed studies of macromolecules, proteins, membranes, and nucleic acids. It includes contributions from experts in the field, covering topics such as tyrosine fluorescence and phosphorescence in proteins and polypeptides, fluorescence dynamics in proteins, tryptophan phosphorescence in proteins, fluorescence studies of nucleic acids, fluorescence in membranes, fluorescence in immunodiagnostic methods, total internal reflection fluorescence, and microparticle fluorescence and energy transfer. Each chapter provides a comprehensive overview of the current state of research and future prospects in these areas.This volume of "Topics in Fluorescence Spectroscopy" focuses on the biochemical applications of fluorescence spectroscopy. Edited by Joseph R. Lakowicz, it is the third in a series of three volumes that cover techniques, principles, and applications in fluorescence spectroscopy. The book highlights recent advancements in time-resolution, data analysis, and instrumentation, which have enabled more detailed studies of macromolecules, proteins, membranes, and nucleic acids. It includes contributions from experts in the field, covering topics such as tyrosine fluorescence and phosphorescence in proteins and polypeptides, fluorescence dynamics in proteins, tryptophan phosphorescence in proteins, fluorescence studies of nucleic acids, fluorescence in membranes, fluorescence in immunodiagnostic methods, total internal reflection fluorescence, and microparticle fluorescence and energy transfer. Each chapter provides a comprehensive overview of the current state of research and future prospects in these areas.