A new RNAi library based on the ORFeome of Caenorhabditis elegans has been developed to improve phenome mapping. The ORFeome is a collection of all open reading frames (ORFs) in the worm genome, and it provides a flexible platform for generating RNAi resources. This library, called ORFeome-RNAi v1.1, contains 11,511 RNAi clones, each targeting a single gene. It targets approximately 55% of the predicted worm ORFeome and includes 1,736 genes not previously targeted by RNAi-by-feeding libraries. This library was used to perform a genome-wide RNAi screen in WT C. elegans hermaphrodites, revealing phenotypes for about 10% of the genes studied. The results show that the ORFeome-RNAi v1.1 library is effective for generating alternative RNAi resources and improving phenome mapping in C. elegans. The study also highlights the importance of using flexible cloning systems to generate RNAi libraries that can be adapted to various experimental conditions and technologies. The ORFeome-RNAi v1.1 library is available for use by the research community and is expected to contribute to a deeper understanding of C. elegans biology.A new RNAi library based on the ORFeome of Caenorhabditis elegans has been developed to improve phenome mapping. The ORFeome is a collection of all open reading frames (ORFs) in the worm genome, and it provides a flexible platform for generating RNAi resources. This library, called ORFeome-RNAi v1.1, contains 11,511 RNAi clones, each targeting a single gene. It targets approximately 55% of the predicted worm ORFeome and includes 1,736 genes not previously targeted by RNAi-by-feeding libraries. This library was used to perform a genome-wide RNAi screen in WT C. elegans hermaphrodites, revealing phenotypes for about 10% of the genes studied. The results show that the ORFeome-RNAi v1.1 library is effective for generating alternative RNAi resources and improving phenome mapping in C. elegans. The study also highlights the importance of using flexible cloning systems to generate RNAi libraries that can be adapted to various experimental conditions and technologies. The ORFeome-RNAi v1.1 library is available for use by the research community and is expected to contribute to a deeper understanding of C. elegans biology.