Transcriptome Changes for Arabidopsis in Response to Salt, Osmotic, and Cold Stress

Transcriptome Changes for Arabidopsis in Response to Salt, Osmotic, and Cold Stress

December 2002 | Joel A. Kreps, Yajun Wu, Hur-Song Chang, Tong Zhu, Xun Wang, and Jeff F. Harper
The study by Kreps et al. (2002) investigates the transcriptome changes in Arabidopsis plants subjected to salt, osmotic, and cold stress. Using a GeneChip microarray, they identified 2,409 genes with a greater than 2-fold change compared to control conditions. The majority of these changes were stimulus-specific, with less than 5% of the changes being shared among all three stress treatments at the 3-hour time point. By 27 hours, the number of shared responses decreased to less than 0.5%. Roots and leaves displayed distinct changes, with less than 14% of cold-specific changes shared between the two tissues. The gene At5g52310 (LTI/COR78) showed the highest induction under all three stress conditions. The study also found that 68% of circadian-controlled genes were linked to stress regulation, suggesting that the circadian clock plays a role in anticipating predictable stresses. The results highlight the complexity of stress responses and the need for further research to understand the biochemical functions of many stress-regulated genes.The study by Kreps et al. (2002) investigates the transcriptome changes in Arabidopsis plants subjected to salt, osmotic, and cold stress. Using a GeneChip microarray, they identified 2,409 genes with a greater than 2-fold change compared to control conditions. The majority of these changes were stimulus-specific, with less than 5% of the changes being shared among all three stress treatments at the 3-hour time point. By 27 hours, the number of shared responses decreased to less than 0.5%. Roots and leaves displayed distinct changes, with less than 14% of cold-specific changes shared between the two tissues. The gene At5g52310 (LTI/COR78) showed the highest induction under all three stress conditions. The study also found that 68% of circadian-controlled genes were linked to stress regulation, suggesting that the circadian clock plays a role in anticipating predictable stresses. The results highlight the complexity of stress responses and the need for further research to understand the biochemical functions of many stress-regulated genes.
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