The article provides an overview of the transport of nanoparticles into plants and the methods used to detect them within plant tissues. It discusses the challenges and prospects associated with nanoparticle delivery, focusing on passive and assisted methods. The barriers to nanoparticle delivery are highlighted, emphasizing the need for innovative approaches, such as using stems as new introduction sites. Recent research has deepened the understanding of nanoparticle-plant interactions, including uptake pathways and translocation mechanisms. The article also emphasizes the importance of multiple detection methods for data validation to enhance the reliability of research findings. Future outlooks include the potential use of alternative introduction sites and the development of improved nanoparticle formulations. By addressing these challenges and fostering multidisciplinary research, the field of nanoparticle transport in plants is poised to make significant contributions to sustainable agriculture and environmental management.The article provides an overview of the transport of nanoparticles into plants and the methods used to detect them within plant tissues. It discusses the challenges and prospects associated with nanoparticle delivery, focusing on passive and assisted methods. The barriers to nanoparticle delivery are highlighted, emphasizing the need for innovative approaches, such as using stems as new introduction sites. Recent research has deepened the understanding of nanoparticle-plant interactions, including uptake pathways and translocation mechanisms. The article also emphasizes the importance of multiple detection methods for data validation to enhance the reliability of research findings. Future outlooks include the potential use of alternative introduction sites and the development of improved nanoparticle formulations. By addressing these challenges and fostering multidisciplinary research, the field of nanoparticle transport in plants is poised to make significant contributions to sustainable agriculture and environmental management.