The transport of nanoparticles into plants is an emerging field with significant applications in agriculture and biotechnology. This review discusses the challenges and prospects of nanoparticle transport in plants, focusing on delivery methods and detection techniques. Passive and assisted delivery methods, including root and leaf introduction sites, are explored, along with their advantages and limitations. Barriers in nanoparticle delivery are highlighted, emphasizing the need for innovative approaches, such as using the stem as a new recognition site, to optimize transport efficiency. Recent research has deepened understanding of nanoparticle interactions with plant tissues and cells, revealing nuanced responses to different nanoparticle types. Detection methods are crucial for studying nanoparticle localization and quantification in plant tissues, with various techniques providing valuable insights. Multiple detection methods are emphasized for data validation to enhance research reliability. Future directions include exploring alternative introduction sites, such as stems, and developing nanoparticle formulations that improve adhesion and penetration. Addressing these challenges and fostering interdisciplinary research can significantly contribute to sustainable agriculture and environmental management. Nanoparticles can be used for nutrient, genetic material, and pesticide delivery, offering benefits in agriculture. However, nanoparticle toxicity, environmental impact, and long-term effects must be considered. Delivery methods include assisted and passive delivery, each with unique advantages and disadvantages. Passive delivery is simpler and more environmentally friendly, with roots and leaves as primary sites. However, natural barriers can hinder nanoparticle delivery. Stems, with their larger vascular system, offer a promising alternative for nanoparticle delivery. Detection methods are critical for understanding nanoparticle interactions with plants, and using multiple methods enhances data reliability. The future of nanoparticle transport in plants involves optimizing delivery methods and understanding nanoparticle effects on plant growth and development.The transport of nanoparticles into plants is an emerging field with significant applications in agriculture and biotechnology. This review discusses the challenges and prospects of nanoparticle transport in plants, focusing on delivery methods and detection techniques. Passive and assisted delivery methods, including root and leaf introduction sites, are explored, along with their advantages and limitations. Barriers in nanoparticle delivery are highlighted, emphasizing the need for innovative approaches, such as using the stem as a new recognition site, to optimize transport efficiency. Recent research has deepened understanding of nanoparticle interactions with plant tissues and cells, revealing nuanced responses to different nanoparticle types. Detection methods are crucial for studying nanoparticle localization and quantification in plant tissues, with various techniques providing valuable insights. Multiple detection methods are emphasized for data validation to enhance research reliability. Future directions include exploring alternative introduction sites, such as stems, and developing nanoparticle formulations that improve adhesion and penetration. Addressing these challenges and fostering interdisciplinary research can significantly contribute to sustainable agriculture and environmental management. Nanoparticles can be used for nutrient, genetic material, and pesticide delivery, offering benefits in agriculture. However, nanoparticle toxicity, environmental impact, and long-term effects must be considered. Delivery methods include assisted and passive delivery, each with unique advantages and disadvantages. Passive delivery is simpler and more environmentally friendly, with roots and leaves as primary sites. However, natural barriers can hinder nanoparticle delivery. Stems, with their larger vascular system, offer a promising alternative for nanoparticle delivery. Detection methods are critical for understanding nanoparticle interactions with plants, and using multiple methods enhances data reliability. The future of nanoparticle transport in plants involves optimizing delivery methods and understanding nanoparticle effects on plant growth and development.