A new method for precise and efficient plant genome engineering, called transposase-assisted target-site integration (TATSI), has been developed. This method combines the natural ability of transposable elements (TEs) to insert DNA into genomes with programmable nucleases like CRISPR-Cas9 or Cas12a. By fusing TE transposase proteins with these nucleases, the system enables targeted insertion of DNA sequences into specific locations in plant genomes. This approach improves the efficiency and accuracy of genome editing compared to existing methods, allowing for the delivery of gene expression cassettes, enhancer elements, and other genetic materials. The study demonstrates the effectiveness of TATSI in model plants like Arabidopsis and in soybean, a major global crop. The system uses the rice mPing transposable element, which can be excised and reinserted into new genomic locations. When combined with programmable nucleases, mPing can be directed to specific sites, enabling precise DNA insertion. The method was tested in Arabidopsis and soybean, showing high rates of targeted insertion with minimal off-target effects. TATSI offers several advantages over existing genome editing techniques, including higher efficiency, accuracy, and the ability to deliver larger genetic cargos. The system can be used to insert genes responsible for traits such as herbicide resistance, which can improve crop yields and reduce production costs. The study also highlights the potential for future improvements, such as increasing the efficiency of TE excision and reducing free transpositions. The research provides a powerful tool for plant genome engineering, enabling the precise modification of plant genomes for agricultural improvement. The method is applicable to a wide range of crops, with the exception of rice, where it may be epigenetically silenced. Overall, TATSI represents a significant advancement in plant genome editing technology, offering a more efficient and accurate alternative to traditional methods.A new method for precise and efficient plant genome engineering, called transposase-assisted target-site integration (TATSI), has been developed. This method combines the natural ability of transposable elements (TEs) to insert DNA into genomes with programmable nucleases like CRISPR-Cas9 or Cas12a. By fusing TE transposase proteins with these nucleases, the system enables targeted insertion of DNA sequences into specific locations in plant genomes. This approach improves the efficiency and accuracy of genome editing compared to existing methods, allowing for the delivery of gene expression cassettes, enhancer elements, and other genetic materials. The study demonstrates the effectiveness of TATSI in model plants like Arabidopsis and in soybean, a major global crop. The system uses the rice mPing transposable element, which can be excised and reinserted into new genomic locations. When combined with programmable nucleases, mPing can be directed to specific sites, enabling precise DNA insertion. The method was tested in Arabidopsis and soybean, showing high rates of targeted insertion with minimal off-target effects. TATSI offers several advantages over existing genome editing techniques, including higher efficiency, accuracy, and the ability to deliver larger genetic cargos. The system can be used to insert genes responsible for traits such as herbicide resistance, which can improve crop yields and reduce production costs. The study also highlights the potential for future improvements, such as increasing the efficiency of TE excision and reducing free transpositions. The research provides a powerful tool for plant genome engineering, enabling the precise modification of plant genomes for agricultural improvement. The method is applicable to a wide range of crops, with the exception of rice, where it may be epigenetically silenced. Overall, TATSI represents a significant advancement in plant genome editing technology, offering a more efficient and accurate alternative to traditional methods.