TreeGraph 2 is a user-friendly, GUI-based graphical editor for phylogenetic trees, designed to combine and visualize evidence from multiple phylogenetic analyses. It allows for the automatic combination of information from different datasets or subsets, helping to identify and graphically present incongruences. The program offers versatile editing and formatting options, such as automatically setting line widths or colors based on node or branch values. Users can import data from various formats, including Newick, Nexus, and phyloXML, and perform operations like tree rerooting, ladderizing, and moving nodes. TreeGraph 2 supports the import of annotations from text files and spreadsheet applications, enabling the display of multiple annotations on nodes. It also provides advanced formatting capabilities, including automatic setting of line widths, text heights, and colors, and supports various graphic output formats such as SVG, PDF, and PNG. The software is written in Java and is platform-independent, making it accessible to a wide range of users. TreeGraph 2 is particularly useful for studies involving extensive gene family datasets and multiple phylogenetic analyses, as it simplifies the process of visualizing and presenting complex phylogenetic data.TreeGraph 2 is a user-friendly, GUI-based graphical editor for phylogenetic trees, designed to combine and visualize evidence from multiple phylogenetic analyses. It allows for the automatic combination of information from different datasets or subsets, helping to identify and graphically present incongruences. The program offers versatile editing and formatting options, such as automatically setting line widths or colors based on node or branch values. Users can import data from various formats, including Newick, Nexus, and phyloXML, and perform operations like tree rerooting, ladderizing, and moving nodes. TreeGraph 2 supports the import of annotations from text files and spreadsheet applications, enabling the display of multiple annotations on nodes. It also provides advanced formatting capabilities, including automatic setting of line widths, text heights, and colors, and supports various graphic output formats such as SVG, PDF, and PNG. The software is written in Java and is platform-independent, making it accessible to a wide range of users. TreeGraph 2 is particularly useful for studies involving extensive gene family datasets and multiple phylogenetic analyses, as it simplifies the process of visualizing and presenting complex phylogenetic data.