This paper presents a method for two-dimensional birefringence imaging in biological tissue using polarization-sensitive optical coherence tomography (PS-OCT). The authors, Johannes F. de Boer, Thomas E. Milner, Martin J. C. van Gemert, and J. Stuart Nelson, describe a technique that measures the polarization state of backscattered light to determine the optical phase delay between fast and slow axes in birefringent materials like bovine tendon. By detecting both polarization states, they can map the change in birefringence with depth, providing rapid non-contact imaging of tissue structural properties. The technique is demonstrated through images of bovine tendon before and after pulsed laser irradiation, showing the loss of birefringence due to thermal damage. The study highlights the potential of PS-OCT for real-time diagnostics in laser therapeutic procedures, particularly in assessing the efficacy of laser therapy based on changes in birefringence.This paper presents a method for two-dimensional birefringence imaging in biological tissue using polarization-sensitive optical coherence tomography (PS-OCT). The authors, Johannes F. de Boer, Thomas E. Milner, Martin J. C. van Gemert, and J. Stuart Nelson, describe a technique that measures the polarization state of backscattered light to determine the optical phase delay between fast and slow axes in birefringent materials like bovine tendon. By detecting both polarization states, they can map the change in birefringence with depth, providing rapid non-contact imaging of tissue structural properties. The technique is demonstrated through images of bovine tendon before and after pulsed laser irradiation, showing the loss of birefringence due to thermal damage. The study highlights the potential of PS-OCT for real-time diagnostics in laser therapeutic procedures, particularly in assessing the efficacy of laser therapy based on changes in birefringence.