This study presents a novel technique, lightwave-driven scanning tunnelling spectroscopy (LW-STS), to directly observe the evolution of a single selenium vacancy in a moiré-distorted WSe₂ monolayer under controlled lattice vibrations. By locally exciting phonon oscillations and taking ultrafast energy-resolved snapshots, the researchers measure the impact of electron-phonon coupling on the isolated defect states. The combination of atomic spatial, sub-picosecond temporal, and millielectronvolt energy resolution provides a comprehensive understanding of complex quantum materials, allowing for the disentanglement of key microscopic interactions. The study reveals that the excitation of an acoustic phonon can adiabatically shift the first defect level on timescales shorter than the oscillation period, with the shift being influenced by a complex interplay of lattice distortions. This work opens new avenues for controlling many-body electronic states and observing moiré exciton trapping and phase transitions in high-temperature superconductivity.This study presents a novel technique, lightwave-driven scanning tunnelling spectroscopy (LW-STS), to directly observe the evolution of a single selenium vacancy in a moiré-distorted WSe₂ monolayer under controlled lattice vibrations. By locally exciting phonon oscillations and taking ultrafast energy-resolved snapshots, the researchers measure the impact of electron-phonon coupling on the isolated defect states. The combination of atomic spatial, sub-picosecond temporal, and millielectronvolt energy resolution provides a comprehensive understanding of complex quantum materials, allowing for the disentanglement of key microscopic interactions. The study reveals that the excitation of an acoustic phonon can adiabatically shift the first defect level on timescales shorter than the oscillation period, with the shift being influenced by a complex interplay of lattice distortions. This work opens new avenues for controlling many-body electronic states and observing moiré exciton trapping and phase transitions in high-temperature superconductivity.