Ligilactobacillus salivarius, formerly known as Lactobacillus salivarius, is a lactic acid bacterium commonly found in the oropharyngeal-gastrointestinal tract (OGT). It has gained significant attention due to its probiotic and functional properties, including strong resistance and adhesion in the OGT, antioxidant and antimicrobial properties, and the ability to produce bacteriocins with antagonistic activity. These characteristics make it a promising candidate for promoting human health. L. salivarius strains have been shown to positively affect the composition of intestinal microbiota, improving metabolic profiling and promoting a healthy internal environment. Recent studies using multi-omics technologies such as genomics, transcriptomics, proteomics, and metabolomics have provided deeper insights into the roles and mechanisms of L. salivarius associated with its functional properties. This review summarizes the probiotic characteristics of L. salivarius, including its interactions with the host microflora, and insights from omics studies. L. salivarius exhibits resistance to acidic pH, bile salts, and other environmental stresses, and has the ability to adhere to the intestinal mucosa. It also possesses antioxidant and antimicrobial properties, and can produce bacteriocins that inhibit pathogenic bacteria. Additionally, L. salivarius strains have been shown to improve the composition of the intestinal microbiota and have beneficial effects on the food industry. The application of multi-omics technologies has enhanced the understanding of the molecular mechanisms underlying the functional and probiotic properties of L. salivarius. This review provides a reference for future research on L. salivarius strains.Ligilactobacillus salivarius, formerly known as Lactobacillus salivarius, is a lactic acid bacterium commonly found in the oropharyngeal-gastrointestinal tract (OGT). It has gained significant attention due to its probiotic and functional properties, including strong resistance and adhesion in the OGT, antioxidant and antimicrobial properties, and the ability to produce bacteriocins with antagonistic activity. These characteristics make it a promising candidate for promoting human health. L. salivarius strains have been shown to positively affect the composition of intestinal microbiota, improving metabolic profiling and promoting a healthy internal environment. Recent studies using multi-omics technologies such as genomics, transcriptomics, proteomics, and metabolomics have provided deeper insights into the roles and mechanisms of L. salivarius associated with its functional properties. This review summarizes the probiotic characteristics of L. salivarius, including its interactions with the host microflora, and insights from omics studies. L. salivarius exhibits resistance to acidic pH, bile salts, and other environmental stresses, and has the ability to adhere to the intestinal mucosa. It also possesses antioxidant and antimicrobial properties, and can produce bacteriocins that inhibit pathogenic bacteria. Additionally, L. salivarius strains have been shown to improve the composition of the intestinal microbiota and have beneficial effects on the food industry. The application of multi-omics technologies has enhanced the understanding of the molecular mechanisms underlying the functional and probiotic properties of L. salivarius. This review provides a reference for future research on L. salivarius strains.