The UniProt Knowledgebase is a comprehensive resource that contains sequences and detailed annotations for over 120 million proteins across all branches of life. This article highlights significant updates made to the resource over the past two years, focusing on expanding the number of Reference Proteomes, particularly viral ones. The UniProt website now includes new data visualizations for subcellular localization, protein structure, and interactions. The UniProt consortium has also improved its automatic annotation pipelines and developed new computational methods for functional annotation. Additionally, the consortium has enhanced its training and outreach efforts, including webinars and online resources, to better serve its users. The UniProt license has been updated to a CC-BY (4.0) to facilitate easier reuse of its data. The article emphasizes the importance of connecting scientific papers to relevant UniProt entries and encourages authors to use specific formatting to facilitate this connection.The UniProt Knowledgebase is a comprehensive resource that contains sequences and detailed annotations for over 120 million proteins across all branches of life. This article highlights significant updates made to the resource over the past two years, focusing on expanding the number of Reference Proteomes, particularly viral ones. The UniProt website now includes new data visualizations for subcellular localization, protein structure, and interactions. The UniProt consortium has also improved its automatic annotation pipelines and developed new computational methods for functional annotation. Additionally, the consortium has enhanced its training and outreach efforts, including webinars and online resources, to better serve its users. The UniProt license has been updated to a CC-BY (4.0) to facilitate easier reuse of its data. The article emphasizes the importance of connecting scientific papers to relevant UniProt entries and encourages authors to use specific formatting to facilitate this connection.