A new technology has been developed to reduce nitrous oxide (N₂O) emissions from farmland by using organic waste as a substrate and vector for N₂O-respiring bacteria selected for their ability to thrive in soil. The study focuses on Cloacibacterium sp. CB-01, a non-denitrifying N₂O-respiring bacterium (NNRB) that effectively reduces N₂O emissions. When grown in biogas digestate, CB-01 reduced N₂O emissions by 50–95% depending on soil type. The strong and long-lasting effect of CB-01 is attributed to its ability to survive in soil, rather than its biokinetic parameters, which were inferior to other N₂O-respiring bacteria. Scaling up the results to the European level, the study estimates that national anthropogenic N₂O emissions could be reduced by 5–20%, with even greater reductions if other organic wastes are included. The study highlights the importance of improving nitrogen-use efficiency in agroecosystems to reduce N₂O emissions. While improving nitrogen-use efficiency can reduce emissions, manipulating soil microorganisms offers even greater potential for substantial reductions. N₂O is produced by various microorganisms, including denitrifying bacteria, ammonia-oxidizing archaea, and abiotic chemical reactions. Denitrifying bacteria can act as sinks, sources, or both for N₂O. The enzyme N₂O reductase (NosZ) is the only biological sink for N₂O in soils, and enhancing its activity can reduce N₂O emissions. The study demonstrates that CB-01, when introduced into soil through digestate, significantly reduces N₂O emissions. Field experiments showed that CB-01 reduced N₂O emissions by up to 95% in some soils. The survival of CB-01 in soil was a key factor in its effectiveness, as it remained active for extended periods. The study also found that CB-01's effect was most pronounced in soils with high N₂O emissions, suggesting that it could be particularly effective in reducing emissions in high-emission areas. The study concludes that the technology has the potential to significantly reduce N₂O emissions, especially when combined with other mitigation strategies. However, further research is needed to optimize the technology and ensure its long-term effectiveness in reducing N₂O emissions. The study also highlights the importance of considering soil type and other environmental factors when implementing this technology. Overall, the study provides a promising approach to reducing N₂O emissions from farmland, which is a major contributor to global warming.A new technology has been developed to reduce nitrous oxide (N₂O) emissions from farmland by using organic waste as a substrate and vector for N₂O-respiring bacteria selected for their ability to thrive in soil. The study focuses on Cloacibacterium sp. CB-01, a non-denitrifying N₂O-respiring bacterium (NNRB) that effectively reduces N₂O emissions. When grown in biogas digestate, CB-01 reduced N₂O emissions by 50–95% depending on soil type. The strong and long-lasting effect of CB-01 is attributed to its ability to survive in soil, rather than its biokinetic parameters, which were inferior to other N₂O-respiring bacteria. Scaling up the results to the European level, the study estimates that national anthropogenic N₂O emissions could be reduced by 5–20%, with even greater reductions if other organic wastes are included. The study highlights the importance of improving nitrogen-use efficiency in agroecosystems to reduce N₂O emissions. While improving nitrogen-use efficiency can reduce emissions, manipulating soil microorganisms offers even greater potential for substantial reductions. N₂O is produced by various microorganisms, including denitrifying bacteria, ammonia-oxidizing archaea, and abiotic chemical reactions. Denitrifying bacteria can act as sinks, sources, or both for N₂O. The enzyme N₂O reductase (NosZ) is the only biological sink for N₂O in soils, and enhancing its activity can reduce N₂O emissions. The study demonstrates that CB-01, when introduced into soil through digestate, significantly reduces N₂O emissions. Field experiments showed that CB-01 reduced N₂O emissions by up to 95% in some soils. The survival of CB-01 in soil was a key factor in its effectiveness, as it remained active for extended periods. The study also found that CB-01's effect was most pronounced in soils with high N₂O emissions, suggesting that it could be particularly effective in reducing emissions in high-emission areas. The study concludes that the technology has the potential to significantly reduce N₂O emissions, especially when combined with other mitigation strategies. However, further research is needed to optimize the technology and ensure its long-term effectiveness in reducing N₂O emissions. The study also highlights the importance of considering soil type and other environmental factors when implementing this technology. Overall, the study provides a promising approach to reducing N₂O emissions from farmland, which is a major contributor to global warming.