This study investigates the use of Raman spectroscopy to assess cadmium toxicity on marine mussels (Mytilus edulis). The research was conducted by a team from Nantes Université, ONIRIS, CNRS, GEPEA, and ISOMer. The aim was to evaluate the reproducibility and variability of Raman spectra in different tissues of mussels exposed to cadmium. The spectral data were pre-processed using Opus software, and heat maps were used to assess the reproducibility of the spectra. The study found that the variability in Raman spectra was greater in the mantle than in the gills. Principal Component Analysis (PCA) was applied to the Raman spectra to separate control and exposed mussels, and the Kruskal Wallis test was used to determine statistical significance. The results showed no significant differences in the gills between control and exposed mussels at two different concentrations of cadmium. The study concluded with thanks to the team members and financial support provided.This study investigates the use of Raman spectroscopy to assess cadmium toxicity on marine mussels (Mytilus edulis). The research was conducted by a team from Nantes Université, ONIRIS, CNRS, GEPEA, and ISOMer. The aim was to evaluate the reproducibility and variability of Raman spectra in different tissues of mussels exposed to cadmium. The spectral data were pre-processed using Opus software, and heat maps were used to assess the reproducibility of the spectra. The study found that the variability in Raman spectra was greater in the mantle than in the gills. Principal Component Analysis (PCA) was applied to the Raman spectra to separate control and exposed mussels, and the Kruskal Wallis test was used to determine statistical significance. The results showed no significant differences in the gills between control and exposed mussels at two different concentrations of cadmium. The study concluded with thanks to the team members and financial support provided.