VFDB is a comprehensive and user-friendly reference database designed to facilitate research on bacterial virulence factors (VFs). The database aims to provide a centralized source of information about VFs from various medically significant bacterial pathogens, including their functional and structural biology, and immunological aspects. VFDB offers a unified platform for storing, searching, retrieving, and updating information on VFs, with features such as browsing by genus or keyword searching, and a BLAST search tool. The database currently includes information on 16 important bacterial pathogens, with plans to expand to include more pathogens in future releases. VFDB is maintained and regularly updated to support the elucidation of pathogenic mechanisms and the development of new approaches for disease treatment and prevention.VFDB is a comprehensive and user-friendly reference database designed to facilitate research on bacterial virulence factors (VFs). The database aims to provide a centralized source of information about VFs from various medically significant bacterial pathogens, including their functional and structural biology, and immunological aspects. VFDB offers a unified platform for storing, searching, retrieving, and updating information on VFs, with features such as browsing by genus or keyword searching, and a BLAST search tool. The database currently includes information on 16 important bacterial pathogens, with plans to expand to include more pathogens in future releases. VFDB is maintained and regularly updated to support the elucidation of pathogenic mechanisms and the development of new approaches for disease treatment and prevention.