The VISTA Enhancer Browser is a comprehensive database that catalogs tissue-specific human enhancers, aiming to address the lack of experimentally characterized enhancer collections. The database integrates experimental data from transgenic mouse assays and computational predictions based on sequence conservation. Over 250 experimentally tested DNA fragments are included, with more than 100 validated as tissue-specific enhancers. Each enhancer entry provides digital images of whole-mount embryo staining and an anatomical description of the reporter gene expression pattern. Users can search for enhancers near specific genes, target particular tissues, or download collections of enhancers with defined tissue specificity or conservation depth. The database is designed to support both experimental and computational studies of enhancer function, facilitating the development of computational methods for enhancer prediction. The VISTA Enhancer Browser is freely accessible at http://enhancer.lbl.gov.The VISTA Enhancer Browser is a comprehensive database that catalogs tissue-specific human enhancers, aiming to address the lack of experimentally characterized enhancer collections. The database integrates experimental data from transgenic mouse assays and computational predictions based on sequence conservation. Over 250 experimentally tested DNA fragments are included, with more than 100 validated as tissue-specific enhancers. Each enhancer entry provides digital images of whole-mount embryo staining and an anatomical description of the reporter gene expression pattern. Users can search for enhancers near specific genes, target particular tissues, or download collections of enhancers with defined tissue specificity or conservation depth. The database is designed to support both experimental and computational studies of enhancer function, facilitating the development of computational methods for enhancer prediction. The VISTA Enhancer Browser is freely accessible at http://enhancer.lbl.gov.