The paper introduces a novel method for subdiffraction imaging called Points Accumulation for Imaging in Nanoscale Topography (PAINT). This method uses fluorescent probes diffusing in solution to target the surface of objects, allowing for the accumulation of probe molecules through collisional flux. By maintaining a low probe density on the object surface, each probe's point-spread function (PSF) can be localized with high precision, enabling the formation of high-resolution images. The method achieves spatial resolutions of approximately 25 nm and can image lipid bilayers, large unilamellar vesicles (LUVs), and other structures. The authors demonstrate the technique using Nile red and transferrin-labeled proteins, showing that it can resolve closely spaced objects and provide detailed structural information. The PAINT method offers a noninvasive, label-free approach to imaging biological structures with subdiffraction resolution, making it a valuable tool for visualizing complex biological processes and structures.The paper introduces a novel method for subdiffraction imaging called Points Accumulation for Imaging in Nanoscale Topography (PAINT). This method uses fluorescent probes diffusing in solution to target the surface of objects, allowing for the accumulation of probe molecules through collisional flux. By maintaining a low probe density on the object surface, each probe's point-spread function (PSF) can be localized with high precision, enabling the formation of high-resolution images. The method achieves spatial resolutions of approximately 25 nm and can image lipid bilayers, large unilamellar vesicles (LUVs), and other structures. The authors demonstrate the technique using Nile red and transferrin-labeled proteins, showing that it can resolve closely spaced objects and provide detailed structural information. The PAINT method offers a noninvasive, label-free approach to imaging biological structures with subdiffraction resolution, making it a valuable tool for visualizing complex biological processes and structures.